Skip to Content
MilliporeSigma
All Photos(4)

Documents

A3428

Sigma-Aldrich

Aprotinin

3-8 TIU/mg solid, lyophilized powder, suitable for cell culture

Synonym(s):

BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

Empirical Formula (Hill Notation):
C284H432N84O79S7
CAS Number:
9087-70-1
Molecular Weight:
6511.44
EC Number:
232-994-9
MDL number:
MFCD00130541
UNSPSC Code:
12352200
eCl@ss:
32190102
NACRES:
NA.32

product name

Aprotinin from bovine lung, lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell culture

biological source

bovine lung

Quality Level

200

product line

BioReagent

form

lyophilized powder

specific activity

3-8 TIU/mg solid

mol wt

~6,500

technique(s)

cell culture | mammalian: suitable

solubility

H2O: 5 mg/mL

UniProt accession no.

P00974

storage temp.

2-8°C

InChI key

ZPNFWUPYTFPOJU-UHFFFAOYSA-N

Gene Information

cow ... PTI(404172)

Looking for similar products? Visit Product Comparison Guide

Application

Aprotinin from bovine lung has been used in insulin and glucagon-like peptide-1 assay. It has also been used a in western blotting.
Aprotinin is largely used as an inhibitor of trypsin.

Biochem/physiol Actions

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
Aprotinin is a natural polypeptide of 58 amino acids. It possesses hemostatic/antiplasmin action and helps to reduce bleeding during surgeries. This prevents the necessity of blood transfusion.

Unit Definition

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.
When benzoyl-L-arginine ethyl ester (BAEE) is used as substrate one BAPNA unit is approx. 45BAEE μmolar units at pH8.0 at 25°C or approx. 9,000 BAEE A253 units at pH7.6 at 25°C.

Preparation Note

This product is a lyophilized powder that has been cell culture tested. It has an activity of 3-7 TIU/mg.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Haemostasis in Spine Surgery, 56-57 (2005)
How the hindgut can cure type 2 diabetes. Ileal transposition improves glucose metabolism and beta-cell function in Goto-kakizaki rats through an enhanced Proglucagon gene expression and L-cell number
Patriti A, et al.
Surgery, 142(1), 74-85 (2007)
Spontaneous epileptiform discharges in a mouse model of Alzheimer's disease are suppressed by antiepileptic drugs that block sodium channels
Ziyatdinova S, et al.
Epilepsy Research, 94(1-2), 75-85 (2011)
Marc R Del Bigio et al.
Cerebrospinal fluid research, 5, 12-12 (2008-07-12)
The cerebral cortex may be compressed in hydrocephalus and some experiments suggest that movement of extracellular substances through the cortex is impaired. We hypothesized that the extracellular compartment is reduced in size and that the composition of the extracellular compartment
David B Chou et al.
Nature biomedical engineering, 4(4), 394-406 (2020-01-29)
The inaccessibility of living bone marrow (BM) hampers the study of its pathophysiology under myelotoxic stress induced by drugs, radiation or genetic mutations. Here, we show that a vascularized human BM-on-a-chip (BM chip) supports the differentiation and maturation of multiple
View All Related Papers

Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

Protocols

Enzymatic Assay of Aprotinin

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service