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A4541

Sigma-Aldrich

Anti-Biotin−Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-Biotin, Anti Biotin Hrp
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:2,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
B3640B7653A6029
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

ascites fluid

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

mouse

biological source

goat

technique(s)

direct ELISA: 1:2,000

technique(s)

immunoelectrophoresis: suitable

technique(s)

capture ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: 1:4,000

technique(s)

direct ELISA: 1:10,000

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

dry ice

clone

polyclonal

clone

polyclonal

clone

BN-34, monoclonal

clone

polyclonal

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General description

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. Goat anti-biotin-peroxidase antibody can be used in many applications where biotin may be introduced as target label.
Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

Immunogen

Biotin-KLH conjugate

Application

Biotin-labeled proteins in U937 and BHK cell lysates were analyzed by western blot using HRP-conjugated goat anti-biotin.
Goat anti-biotin-peroxidase antibody can be used for direct ELISA assays at a dilution of 1:2,000.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Preparation Note

Prepared by the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation markEnvironment

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Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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