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A3151

Sigma-Aldrich

Avidin–Peroxidase

lyophilized powder

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Synonym(s):
Avidin-POD
MDL number:
NACRES:
NA.46

biological source

avidin from egg white
enzyme from horseradish

Quality Level

conjugate

peroxidase conjugate

form

lyophilized powder

composition

Protein, ≥70% E1%/280

extent of labeling

0.7-2.0 mol peroxidase per mol avidin

technique(s)

direct ELISA: 1:50,000

solubility

H2O: soluble 1 mg/mL
PBS: soluble

storage temp.

−20°C

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A7294A2901A2050
Sigma-Aldrich

Sigma-Aldrich

A3151

Avidin–Peroxidase

conjugate

peroxidase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

form

lyophilized powder

form

buffered aqueous solution

form

lyophilized powder

form

buffered aqueous solution

technique(s)

direct ELISA: 1:50,000

technique(s)

direct ELISA: 1:70,000, western blot: 1:150,000-1:300,000 using using β-actin in total cell extract of HeLa cells (5-10 μg per lane

technique(s)

immunohistochemistry: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL, indirect immunofluorescence: 1:200

solubility

H2O: soluble 1 mg/mL, PBS: soluble

solubility

-

solubility

-

solubility

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

Application

Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding and OVA-IgG1.
To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

Physical form

Lyophilized powder containing citrate buffer.

Preparation Note

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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