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A8917

Sigma-Aldrich

Anti-Bovine IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Synonym(s):
Rabbit Anti-Bovine IgG (whole molecule)−HRP
MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

bovine

technique(s)

direct ELISA: 1:20,000
dot blot: 1:80,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
A5295A8919A5545
technique(s)

direct ELISA: 1:20,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000, dot blot: 1:80,000

technique(s)

direct ELISA: 1:20,000, dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:2,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot (chemiluminescent): 1:160,000

technique(s)

direct ELISA: 1:50,000-1:80,000, dot blot: 1:50,000-1:100,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400-1:800

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

species reactivity

bovine

species reactivity

bovine

species reactivity

-

species reactivity

guinea pig

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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General description

Immunoglobulin G (IgG) contributes to 10−20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical κ or λ light (L) chains of 50 kDa and 25 kDa, respectively.

Immunogen

purified bovine IgG

Application

Anti-Bovine IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in:
  • Dot- enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunohistology

Rabbit Anti-Bovine IgG (whole molecule)-Peroxidase antibody has been used for western blot and ELISA. The antibody can also be used for dot blot (1:80,000) and immunohistochemistry (1:1,000).

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Development of Rapid Diagnostic Kit for Identification of Hanwoo (Korean Native Cattle) Brand Meat by Detecting BIO-TAG
Baek KH, et al.
Korean journal for food science of animal resources, 34(3), 339-339 (2014)
Comparison of crude and excretory/secretory antigens for the diagnosis of Fasciola hepatica in sheep by western blotting.
Kara, M. and Kircali, F.
Turkish Journal of Veterinary and Animal Sciences, 28, 943-949 (2004)
Investigation of antigenic specificity against Cysticercus tenuicollis cyst fluid antigen in dogs experimentally infected with Taenia hydatigena
Kara M and Douganay A
Turkish Journal of Veterinary and Animal Sciences, 29, 835-840 (2005)
Bárbara Fernández et al.
Veterinary medicine international, 2012, 145318-145318 (2012-07-14)
Johne's Disease or Paratuberculosis is a chronic granulomatous enteritis disease affecting ruminants. Detection of subclinically infected animals is difficult, hampering the control of this disease. The aim of this work was to evaluate the performance of detection of IgG isotypes
George C Russell et al.
Journal of virological methods, 299, 114329-114329 (2021-10-16)
The minor capsid protein of ovine herpesvirus 2, identified as a potential antigen for serological testing, was over-expressed and purified to allow its assessment in ELISA. The corresponding gene sequence (OvHV-2 orf65, Ov65) was modified to incorporate epitope tags and

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