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B6901

Sigma-Aldrich

Monoclonal Anti-Bovine IgG antibody produced in mouse

clone BG-18, ascites fluid

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Synonym(s):
Monoclonal Anti-Bovine IgG
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

BG-18, monoclonal

contains

15 mM sodium azide

species reactivity

bovine (non-reactive to IgM)

should not react with

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

technique(s)

dot blot: suitable
indirect ELISA: 1:1000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
B8400I6137A7554
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

bovine (non-reactive to IgM)

species reactivity

-

species reactivity

-

species reactivity

bovine (non-reactive to IgM)

technique(s)

dot blot: suitable, indirect ELISA: 1:1000

technique(s)

capture ELISA: suitable, dot blot: suitable, indirect ELISA: 1:1,000, western blot: suitable

technique(s)

capture ELISA: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 1:4,000, western blot: suitable

technique(s)

direct ELISA: 1:25,000, dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG, western blot (chemiluminescent): 1:100,000

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

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General description

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.
The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction. IgG (immunoglobulin G) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical γ or γ light (L) chains of 50 kDa and 25 kDa, respectively.

Specificity

bovine IgG1 and IgG2

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG antibody has been used in indirect immunoperoxidase staining.
Monoclonal Anti-Bovine IgG antibody produced in mouse is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. Monoclonal Anti-Bovine IgG antibody produced in mouse was used as an isotype control in ChIP assay.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical form

Ascites fluid containing 0.1% sodium azide

Storage and Stability

For continuous use, store at 2-8°C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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