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A7554

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Monoclonal Anti-Bovine IgG−Alkaline Phosphatase antibody produced in mouse

clone BG-18, purified immunoglobulin, buffered aqueous glycerol solution

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Synonym(s):
Monoclonal Anti-Bovine IgG
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

BG-18, monoclonal

form

buffered aqueous glycerol solution

species reactivity

bovine (non-reactive to IgM)

should not react with

pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat

technique(s)

direct ELISA: 1:25,000
dot blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG
western blot (chemiluminescent): 1:100,000

isotype

IgG1

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
A2556B6901A2306
technique(s)

direct ELISA: 1:25,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:75 using using rabbit spleen sections and bovine anti-rabbit IgG, dot blot: suitable, western blot (chemiluminescent): 1:100,000

technique(s)

direct ELISA: 1:50,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100, western blot: 1:200,000-1:400,000 using total cell extract of HeLa cells

technique(s)

dot blot: suitable, indirect ELISA: 1:1000

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 using human tonsil and Cat. No. I8635 as the primary antibody, western blot: 1:160,000-1:320,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per well)

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

unconjugated

conjugate

alkaline phosphatase conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

purified immunoglobulin

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

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General description

Monoclonal Anti-Bovine IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.

Specificity

bovine IgG1 and IgG2

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG?Alkaline Phosphatase antibody produced in mouse has been used in:
  • western blotting
  • enzyme linked immunosorbent assay (ELISA)
  • dot blot
  • immunohistochemistry

Biochem/physiol Actions

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin. Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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