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I6137

Sigma-Aldrich

Monoclonal Anti-Bovine IgM antibody produced in mouse

clone BM-23, ascites fluid

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MDL number:
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

BM-23, monoclonal

contains

15 mM sodium azide

technique(s)

capture ELISA: suitable
immunoprecipitation (IP): suitable
indirect ELISA: 1:4,000
western blot: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
B8400B6901R0886
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

capture ELISA: suitable, indirect ELISA: 1:4,000, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

capture ELISA: suitable, dot blot: suitable, indirect ELISA: 1:1,000, western blot: suitable

technique(s)

dot blot: suitable, indirect ELISA: 1:1000

technique(s)

capture ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable, indirect ELISA: 1:1,000, western blot: suitable

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

isotype

IgG1

isotype

IgG1

isotype

IgG1

isotype

IgG1

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

Bovine IgMs are glycoprotein antibodies that regulate antigen binding during immunological responses. These antibodies have three isotypes, namely, IgMa, IgMb and IgMc. Structural restraints on movement enhance the complement-fixing functions of bovine IgMs . Monoclonal Anti-Bovine IgM antibody detects an epitope on the heavy chain of bovine IgM. The antibody reacts with bovine serum, but does not react with sera from human, baboon, marmoset, gibbon, rhesus, hamster, rabbit, goat, pig, dog, rat, turkey, chicken, or catfish. Furthermore, the product does not bind to native or reduced bovine light chains.
Monoclonal Anti-Bovine IgM (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgM is highly conserved antibody in vertebrates and is expressed early during immune response. It exists as pentamer and is secreted by peritoneal B cells.

Immunogen

Purified bovine IgM

Application

Monoclonal Anti-Bovine IgM antibody is suitable for use in indirect ELISA (1:1,000), western blot, immunoprecipitation and ELISA.

Biochem/physiol Actions

IgM plays a key role in engulfing of the of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Escherichia coli O157: H7 and other Shiga toxin-producing E. coli in white veal calves
Cristancho L, et al.
Veterinary Microbiology, 126, 200-209 (2008)
Amy S Austin et al.
Journal of immunology (Baltimore, Md. : 1950), 171(3), 1336-1342 (2003-07-23)
IgA is the predominant Ig isotype in mucosal secretions and thus plays a pivotal role in host defense. The mechanisms by which IgA expression is regulated may differ among species and involve multiple pathways. Various cytokines and costimulators have been
Hilary E Kennedy et al.
Infection and immunity, 70(3), 1488-1500 (2002-02-21)
It is accepted that cell-mediated immune responses predominate in mycobacterial infections. Many studies have shown that CD4(+) T cells produce Th1 cytokines, such as gamma interferon (IFN-gamma), in response to mycobacterial antigens and that the cytolytic activity of CD8(+) cells
Surinder S Saini et al.
International immunology, 15(7), 845-853 (2003-06-17)
Naturally occurring antibody repertoires of cattle (Bos taurus) include a group of IgMlambda antibodies with exceptionally long complementarity-determining region 3 of the heavy chain (CDR3H) segments, containing multiple Cys residues. These massive CDR3H segments will greatly influence the tertiary and
S S Saini et al.
Molecular immunology, 38(5), 389-396 (2001-10-31)
The structure of IgM determined from two cDNAs isolated from a Holstein (BLV7G1) and an Angus x Hereford cross-bred (B5D8) cow reveals high sequence similarity both at nucleotide (98.7%) and amino acid (97.9%) level and is closest to sheep (89.4%).

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