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FilBridge ELISA Kit

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antibody form

IgG fraction of antiserum

species reactivity

human (Human IgG)

shipped in

wet ice

storage temp.


General description

A Protein Conformational Array (PCA) in a sandwich ELISA format where the plate is coated with a panel of antibodies raised against peptides derived from the full length protein sequence of human GCSF. Taken individually, each of these antibodies is strongly antigenic to the peptide sequence that was used in its production. However, when these peptides are incorporated into a full length correctly folded protein, the antigenicity of many of them is masked by the three dimensional structure of the protein and a basal level of epitopes are detected by this assay. The result is a histogram which can be likened to a ‘fingerprint′ for correctly folded Filgrastim. For an Filgrastim Biosimilar, if the protein is correctly folded and modified, the ‘fingerprint′ will match that of Filgrastim. If it is not correctly folded, previously masked peptide sequences will be exposed and will be recognized by the antibody made to that exposed sequence. In this way, changes in the ‘fingerprint′ generated by the ELISA will point out differences between the Biosimilar and authentic Filgrastim.


For research use only. Not for use in diagnostic procedures.





Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class

8A - Combustible, corrosive hazardous materials



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