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form
liquid
packaging
pkg of 250 mL (AC-60250 (HighPrep™ PCR (250 mL)))
pkg of 5 mL (AC-60005 (HighPrep™ PCR (5 mL)))
pkg of 50 mL (AC-60050 (HighPrep™ PCR (50 mL)))
pkg of 500 mL (AC-60500 (HighPrep™ PCR (500 mL)))
storage condition
do not freeze
storage temp.
2-8°C
General description
The HighPrep™ PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.
Application
- Post-PCR and post-enzymatic reaction clean-up
- NGS library preparation
- Amplicon size selection for NGS
- Microarrays
- PCR
- Sanger sequencing
- Restriction enzyme digestions, adapter ligations
- Cloning
Features and Benefits
- High recovery of >100 bp amplicons
- Adaptable to high throughput liquid handling systems
- Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
- No centrifugation or filtration leads to higher and purer yields
Storage and Stability
Legal Information
Disclaimer
signalword
Warning
hcodes
Hazard Classifications
Repr. 2
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Protocols
HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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