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HighPrep PCR Clean-up System

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Synonym(s):
PCR clean up kit

form

liquid

packaging

pkg of 250 mL (AC-60250 (HighPrep PCR (250 mL)))
pkg of 5 mL (AC-60005 (HighPrep PCR (5 mL)))
pkg of 50 mL (AC-60050 (HighPrep PCR (50 mL)))
pkg of 500 mL (AC-60500 (HighPrep PCR (500 mL)))

storage condition

do not freeze

storage temp.

2-8°C

General description

Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.

The HighPrep PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.

Application

HighPrep PCR Clean-up System may be used in:
  • Post-PCR and post-enzymatic reaction clean-up
  • NGS library preparation
  • Amplicon size selection for NGS
  • Microarrays
  • PCR
  • Sanger sequencing
  • Restriction enzyme digestions, adapter ligations
  • Cloning

Features and Benefits

  • High recovery of >100 bp amplicons
  • Adaptable to high throughput liquid handling systems
  • Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
  • No centrifugation or filtration leads to higher and purer yields

Storage and Stability

14 months when stored as directed

Legal Information

HiPrep is a trademark of Cytiva
HighPrep is a trademark of MagBio Genomics, Inc.

Disclaimer

For Research Use Only. Not for Use in Diagnostic Procedures.

Pictograms

Health hazard

Signal Word

Warning

Hazard Statements

Hazard Classifications

Repr. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

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In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

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Soil bacterial diversity under conservation agriculture-based cereal systems in Indo-Gangetic Plains
Choudhary M, et al.
3 Biotech, 8, 304-304 (2018)
Vaishali Sah et al.
Gene, 731, 144356-144356 (2020-01-15)
The genetic basis of differential host immune response vis-à-vis transcriptome profile was explored in PBMCs of indigenous (Ghurrah) and crossbred pigs after classical swine fever vaccination and in monocyte derived macrophages (MDMs) challenged with virulent classical swine fever (CSF) virus.
N Salvetat et al.
Journal of neurovirology, 25(6), 825-836 (2019-07-25)
Treatment-emergent depression is a common complication in patients with chronic hepatitis C virus (HCV) infection undergoing antiviral combination therapy with IFN-α and ribavirin. It has recently been shown that changes in A-to-I RNA editing rates are associated with various pathologies
Deep sequencing of near full-length HIV-1 genomes from plasma identifies circulating subtype C and infrequent occurrence of AC recombinant form in Southern India}
Alampalli SV, et al.
PLoS ONE, 17 (2017)
Signature of genome wide gene expression in classical swine fever virus infected macrophages and PBMCs of indigenous vis-a-vis crossbred pigs
Sah V, et al
Gene, 144356-144356 (2020)

Protocols

HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service