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B1125

Sigma-Aldrich

Bathocuproinedisulfonic acid disodium salt

for spectrophotometric det. of Cu, Fe

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Synonym(s):
2,9-Dimethyl-4,7-diphenyl-1,10-phenantrolinedisulfonic acid disodium salt, Disodium bathocuproinedisulfonate
Empirical Formula (Hill Notation):
C26H18N2Na2O6S2
CAS Number:
Molecular Weight:
564.54
NACRES:
NA.47

Assay

7.2-9.1% dry basis (ICP)

form

powder

quality

for spectrophotometric det. of Cu, Fe

solubility

H2O: 50 mg/mL

suitability

suitable for determination of copper

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C26H20N2O6S2.2Na/c1-15-25(35(29,30)31)21(17-9-5-3-6-10-17)19-13-14-20-22(18-11-7-4-8-12-18)26(36(32,33)34)16(2)28-24(20)23(19)27-15;;/h3-14H,1-2H3,(H,29,30,31)(H,32,33,34);;/q;2*+1/p-2

InChI key

RNGKZLRAVYPLJC-UHFFFAOYSA-L

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This Item
D8284D1878230901
form

powder

form

powder

form

powder

form

powder, crystals or chunks

solubility

H2O: 50 mg/mL

solubility

0.5% sodium bicarbonate: 50 mg/mL

solubility

H2O: soluble 10 mg/mL, clear, blue to very deep blue

solubility

-

suitability

suitable for determination of copper

suitability

-

suitability

-

suitability

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
food and beverages
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

2-8°C

storage temp.

room temp

storage temp.

room temp

General description

Bathocuproinedisulfonic acid is a strong copper chelator. It is used for the determination of copper and iron in biological samples.

Application

  • Bathocuproinedisulfonic acid disodium salt has been used as a copper chelator for specific assay in Arabidopsis seedlings.
  • It has been used as an electron chain-associated free radical production inhibitor for superoxide dismutase assay in cells.
  • It has been added to the culture media of trypanosomes as a copper chelator for the stabilization of cysteine.

Other Notes

Mixture of isomers

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue.
Weydert CJ and Cullen JJ
Nature Protocols, 5, 51-66 (2010)
Shinya Ogawa et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 909, 34-36 (2012-11-17)
HPLC eluent systems employing acetonitrile and methanol were evaluated for the quantitation of glutathione (GSH) and phytochelatin (PC(n)), a family of peptides implicated in heavy-metal detoxification in higher plants. The detection system is based on the dequenching of copper(I)-bathocuproine disulfonate
Shinya Ogawa et al.
Analytical chemistry, 81(21), 9199-9200 (2009-09-30)
Reoptimization of analytical conditions was performed for a high-performance liquid chromatographic (HPLC) detection system for Cu(I) chelators based on the dequenching of Cu(I)-bathocuproine disulfonate complexes that occurs in the presence of Cu(I) chelators. The revision corrects for emission and excitation
Tomoo Shirabe et al.
Analytical chemistry, 80(23), 9360-9362 (2009-06-25)
A novel method has been devised for the determination of phytochelatins (PCs), heavy-metal-tolerant peptides produced by higher plants and algae. The method is based on the facts that fluorescence of bathocuproine disulfonate (BCS) is quenched by Cu(I) ions as a
Simple procedure for the single sample determination of serum copper and iron.
B ZAK
Clinica chimica acta; international journal of clinical chemistry, 3(4), 328-334 (1958-07-01)

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