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B2518

Sigma-Aldrich

Albumin, Acetylated from bovine serum

lyophilized powder, suitable for microbiology

Synonym(s):

Ac-BSA, Acetyl BSA, Acetylated BSA

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.31

biological source

bovine

Quality Level

form

lyophilized powder

purified by

heat shock fractionation

origin

USA origin

technique(s)

Southern blotting: suitable
blocking: suitable
cell culture | mammalian: suitable

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

pH

7

suitability

suitable for microbiology

UniProt accession no.

foreign activity

DNase free
DNase, RNase, NICKase, protease, none detected
NICKase free
Protease free
RNase free

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Native bovine serum albumin (BSA) is a 66 kDa protein, which belongs to the family of serum albumin. It consists of three domains (I, II and III) and is the major protein found in circulatory system. Native BSA is an α-helical, globular and non-glycosylated protein, with 17-disulfide bonds.

Acetylation of bovine serum albumin modifies various residues, most notably lysine (Lys), and also serine (Ser) and threonine (Thr) to lesser extents. MALDI-TOF mass spectrometry studies on acetylated BSA have indicated a MW value about 3000 Da higher compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. Acetylation of BSA also modifies parallel amino acid residues in any trace contaminating nucleases in the BSA, which inactivates that trace nuclease content. Thus acetylated BSA can be used in molecular biology applications where trace nuclease activity must be minimized.

Application

Acetylated bovine serum albumin has been used:
  • as a component of protease buffer for protease inhibition assay
  • as a blocking agent in the preparation of cDNA glass array
  • to select hybridoma antibodies sensitive to M-ficolin epitopes by immunofluorometric assay
This B2518 acetylated BSA product has been cited in various publications and protocols, such as the following:






* As an electrophoresis standard: Lindberg, T. et al., J. Pediatr. Gastroenterol. Nutr., 27(1), 30-36 (1998)




* In in situ end-labeling buffer for labeling of DNA fragments: Save, V. et al., ”Detecting and Quantifying Apoptosis in Tissue Sections”, in Apoptosis Methods and Protocols (H.J.M. Brady, ed.). Methods Mol. Biol., Vol. 282, pp. 67-84 (2004)




* In EMSA studies on nucleocytoplasmic shuttling: Ghosh, C.C. et al., “Analysis of Nucleocytoplasmic Shuttling of NF?B Proteins in Human Leukocytes”, in Membrane Trafficking (A. Vancura, ed.). Methods Mol. Biol., Vol. 457, pp. 279-292 (2008)




* In selection buffer for ribosome display studies: Larman, H.B. et al., Nat. Protoc., 9(1), 90-103 (2014)
Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.

Biochem/physiol Actions

Native bovine serum albumin (BSA) acts as a transporter of drugs, hormones and fatty acids. It has calcium binding sites and regulates metal homeostasis.

Features and Benefits

  • Acetylated to inactivate trace nucleases
  • DNase free / Exonuclease free
  • Nickase free / Endonuclease free
  • RNase free
  • Protease free

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Cluster cytometry for high-capacity bioanalysis
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Ficolin-3 deficiency is caused by a mutation (+1637delC) in the FCN3 gene. It is a rare condition and has been associated with both infection and autoimmune disease including systemic lupus erythematosus (SLE). Here we investigated if ficolin-3 deficiency is more
S A Fiscus et al.
Methods in molecular medicine, 20, 209-216 (1999-01-01)
Semen is the major vehicle for the sexual transmission of HIV-1. The ability to isolate infectious HIV from the semen and to quantitate viral burden in the form of cell-free or cell-associated HIV-1 RNA in semen are important for epidemiologic
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Myeloid differentiation primary response protein 88 (MYD88) is a critical universal adapter that transduces signaling from Toll-like and interleukin receptors to downstream nuclear factor-κB (NF-κB). MYD88L265P (leucine changed to proline at position 265) is a gain-of-function mutation that occurs frequently
Identification of genes regulated by interleukin-1beta in human endometrial stromal cells
Rossi M, et al.
Reproduction (Cambridge, England), 130(5), 721-729 (2005)

Protocols

Preparation for biodegradable nanoparticles and their use in transfection protocols .

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