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B4261

Sigma-Aldrich

Biocytin

≥98% (TLC)

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Synonym(s):
Bct, Nε-(+)-Biotinyl-L-lysine
Empirical Formula (Hill Notation):
C16H28N4O4S
CAS Number:
Molecular Weight:
372.48
Beilstein/REAXYS Number:
97197
MDL number:
PubChem Substance ID:
NACRES:
NA.26

assay

≥98% (TLC)

form

powder

optical activity

[α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.)

technique(s)

drug transporter assay: suitable

color

white to off-white

mp

~245 °C (dec.) (lit.)

storage temp.

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NCCCC[C@H](N)C(O)=O)[C@@]1([H])NC(=O)N2

InChI

1S/C16H28N4O4S/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24)/t10-,11-,12-,14-/m0/s1

InChI key

BAQMYDQNMFBZNA-MNXVOIDGSA-N

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1 of 4

This Item
B1758A2010A3626
vibrant-m

B4261

Biocytin

vibrant-m

B1758

Biocytin hydrochloride

vibrant-m

A2010

Nα-Acetyl-L-lysine

vibrant-m

A3626

Nα-Acetyl-L-ornithine

form

powder

form

powder

form

powder

form

powder

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥98% (TLC)

assay

≥98% (TLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mp

~245 °C (dec.) (lit.)

mp

-

mp

256-258 °C (dec.) (lit.)

mp

-

optical activity

[α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.)

optical activity

-

optical activity

-

optical activity

-

Application

Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s), to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues. Biocytin has been used to label neurons, medium spiny neurons and optic nerves.

Biochem/physiol Actions

Biocytin is an adduct of lysine and biotin. It is hydrolyzed by biotinidase, which acts on peptide-incorporated biotin or free biotin. Biocytin acts as a coenzyme.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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