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B7639

Sigma-Aldrich

(+)-Biotin hydrazide

≥97% (TLC), powder

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About This Item

Empirical Formula (Hill Notation):
C10H18N4O2S
CAS Number:
Molecular Weight:
258.34
Beilstein/REAXYS Number:
28347
MDL number:
UNSPSC Code:
12352203
PubChem Substance ID:
NACRES:
NA.46

Quality Level

assay

≥97% (TLC)

form

powder

solubility

DMSO: ≤20 mg/mL

storage temp.

2-8°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)NN)[C@@]1([H])NC(=O)N2

InChI

1S/C10H18N4O2S/c11-14-8(15)4-2-1-3-7-9-6(5-17-7)12-10(16)13-9/h6-7,9H,1-5,11H2,(H,14,15)(H2,12,13,16)/t6-,7-,9-/m0/s1

InChI key

KOZWHQPRAOJMBN-ZKWXMUAHSA-N

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General description

Biotin hydrazide is a biotinylation reagent used to biotinylate glycoproteins with their sugar moieties. Biotin hydrazide can be used to prepare biotin-conjugated alginate. It can also be used for covalent attachment to PAAc via carbodi-imide cross linking.

Application

(+)-Biotin hydrazide has been used:
  • the modification of alginate
  • for the labelling of mitochondrial proteins from non-muscle tissues
  • as a component of glycoprotein staining solution
  • in periodic acid-biotin-hydrazide (PABH) assay for mucins
  • for labeling surface functional groups, biologically active molecules such as antibodies, lectins, sugars, nucleic acids or molecules with free carboxylic or keto groups.
  • for coupling to glycoproteins through the carbohydrate by hydrazone formation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Related product

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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Boris Polyak et al.
Biomacromolecules, 5(2), 389-396 (2004-03-09)
Biotin was covalently coupled with alginate in an aqueous-phase reaction by means of carbodiimide-mediated activation chemistry to provide a biotin-alginate conjugate for subsequent use in biosensor applications. The synthetic procedure was optimized with respect to pH of the reaction medium
Studying mucin secretion from human bronchial epithelial cell primary cultures
Mucins, 259-277 (2012)
Ajay M Shah et al.
Analytical chemistry, 84(8), 3682-3688 (2012-03-15)
Microfluidic systems for affinity-based cell isolation have emerged as a promising approach for the isolation of specific cells from complex matrices (i.e., circulating tumor cells in whole blood). However, these technologies remain limited by the lack of reliable methods for
Suppressive Effect of the o-Amylase Inhibitor Albumin from Buckwheat (Fagopyrum esculentum Moench) on Postprandial Hyperglycaemia
Impact of Bioactive Peptides on Human Health, 157-157 (2016)
Enhanced capture and release of circulating tumor cells using hollow glass microspheres with a nanostructured surface
Dong Z, et al.
Nanoscale, 10(35), 16795-16804 (2018)

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