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B8631

Sigma-Aldrich

Bile extract porcine

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Synonym(s):
Bile acids, Hog Bile Powder
CAS Number:
EC Number:
MDL number:
NACRES:
NA.25

biological source

Porcine

form

powder

technique(s)

microbiological culture: suitable

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This Item
B8381P3292I5523
Bile extract porcine

Sigma-Aldrich

B8631

Bile extract porcine

form

powder

form

powder

form

powder

form

powder

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Bile extract porcine has been used:
  • as a supplement in brain heart infusion (BHI) medium to culture Listeria monocytogenes LO28
  • to investigate the survival of probiotic bacteria through the gastric-small intestine
  • as a supplement in BHI mediato study the effect of oxygen on bile resistance in Listeria monocytogenes

Biochem/physiol Actions

Bile from bovine and ovine can be used in bacteriological research to investigate bile salt adaptation of bacteria such as Lactobacillus.

Quality

Contains glycine and taurine conjugates of hyodeoxycholic acid and other bile salts.

Testing Method

Chemical derivatization and HPLC have been proven for separation and identification of complex mixtures of bile acids and bile acid conjugates.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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This study was undertaken to investigate the impact of culture pH (4.5-6.5) and temperature (32-37 °C) on the stress resilience of Lactobacillus reuteri DSM 17938 during freeze-drying and post freeze-drying exposure to low pH (pH 2) and bile salts. Response-surface methodology
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