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B8895

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

biotin conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct ELISA: 1:200,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800
western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
B3275B7389B7151
conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

conjugate

biotin conjugate

technique(s)

direct ELISA: 1:200,000, western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800

technique(s)

direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500, western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)

technique(s)

direct ELISA: 1:20,000

technique(s)

direct ELISA: 1:200,000-1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:400,000-1:800,000 using total cell extract of HeLa cells

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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General description

IgG antibodies regulate several functions such as phagocytosis and complement activation. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule)–biotin antibody can be used in immunohistochemistry. The product shows minimal interspecies cross reactivity to human IgG hence can be used in enzyme immunoassays or dot blot in presence of human IgG. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically to all rabbit immunoglobulin but has no reactivity with human IgG.
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.

Specificity

Binds all rabbit Igs.

Immunogen

Purified rabbit IgG

Application

Anti-Rabbit IgG (whole molecule)-Biotin antibody has been used in
  • Immunofluorescence
  • Immunohistochemistry
  • Western blot
  • Direct enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Avidin, Streptavidin or ExtrAvidine show strong affinity interaction with biotin rendering the biotin-avidin system an extremely effective tool in molecular biology, protein chemistry and immunology. The high specificity of the biotinylated antibody to rabbit IgG and the stability of the biotin-avidin complex has been exploited in variety of reagents (Avidin, Streptavidin or ExtrAvidin conjugated to FITC, TRITC, Peroxidase or Alkaline Phosphatase), for the detection and quantitation of rabbit IgG.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Preparation Note

Adsorbed to reduce background staining with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Feng Yang et al.
Cancer research, 65(19), 8887-8895 (2005-10-06)
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<BIG>Jiang T, et al.</BIG>
Cellular Physiology and Biochemistry, 43, 1703-1717 (2017)
Jeffery M Vahrenkamp et al.
Cell reports, 22(11), 2995-3005 (2018-03-15)
Steroid hormone receptors are simultaneously active in many tissues and are capable of altering each other's function. Estrogen receptor α (ER) and glucocorticoid receptor (GR) are expressed in the uterus, and their ligands have opposing effects on uterine growth. In
Ting Jiang et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 43(4), 1703-1717 (2017-10-19)
Previous studies have demonstrated that leonurine, a unique alkaloid compound of Herba leonuri, can exert anti-oxidative and anti-inflammatory effects on the development of atherosclerosis (AS). This study was designed to investigate the effects of leonurine on cholesterol efflux from THP-1
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<BIG>Lei L, et al.</BIG>
Sensors and Actuators B, Chemical, 273, 35-40 (2018)

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