B8520
Anti-Mouse IgG (whole molecule)−Biotin antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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About This Item
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biological source
rabbit
Quality Level
conjugate
biotin conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:200,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
western blot: 1:400,000-1:800,000
storage temp.
−20°C
target post-translational modification
unmodified
General description
Immunoglobulins (Igs) belongs to the immunoglobulin super-family. There are five different types of Immunoglobulins. Each immunoglobulin has two heavy (H) and two light (L) chains held together by disulfide linkages. Each light comprises of one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region. IgG is abundant immunoglobulin in human serum.
Immunogen
Purified mouse IgG
Application
Anti-Mouse IgG (whole molecule)-Biotin antibody has been used in
- immunofluorescence
- immunoblot
- direct enzyme linked immunosorbent assay (ELISA)
- serotyping assay
- immunohistochemistry
Biochem/physiol Actions
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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