C0984

Creatine Kinase MB Fraction from human heart

≥70% (SDS-PAGE), liquid

• Synonym(s): CK MB Fraction
• MDL number: MFCD00151686
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: human heart
• Quality Level: 200
• assay: ≥70% (SDS-PAGE)
• form: liquid
• concentration: ≥0.5 mg/mL
• technique(s): IR spectroscopy: suitable
• suitability: suitable for tissue
• UniProt accession no.: P06732; P12277
• application(s): pharmaceutical
• storage temp.: −20°C

Description

General description

Research area: Cell Signaling
Creatine Kinase (CK) comprises M and B subunit and is a dimeric molecule. It is one among the isoenzymes of CK. The enzyme is majorly present in the myocardium.[1]

Application

Creatine Kinase MB Fraction from human heart has been used:

• as a diagnostic marker for acute myocardial infarction.[2]
• to identify overlapping absorbance peaks between blood and the cardiac biomarkers.[3]
• to study the phosphorylation pathway of dioxolane guanosine (DXG) in vivo, a potential treatment of HIV-1 infection.[4]

Biochem/physiol Actions

Creatine Kinase (CK) catalyzes the formation of creatinine from creatine phosphate.[1] The enzyme is associated with coronary performance. Elevated serum levels of CK-MB are prime indicators of myocardial cellular injury.[1] It is regarded as a “gold standard” for myocardial infarction diagnosis.[1] The levels of cardiac troponin I (cTnI) or cardiac troponin T also correlate to higher CK-MB) levels.[5] CK-MB levels may also be elevated in chronic renal failure.[6]

Components

Solution in 5 mM sodium succinate, 10 mM sodium chloride, 1 mM EDTA, 5 mM β-mercaptoethanol, 50% glycerol, pH 7.0.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable