MilliporeSigma
All Photos(3)

Documents

C1801

Sigma-Aldrich

Monoclonal Anti-Cytokeratin, pan antibody produced in mouse

clone PCK-26, ascites fluid

Sign Into View Organizational & Contract Pricing

Synonym(s):
Monoclonal Anti-pan-Cytokeratin

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

PCK-26, monoclonal

contains

15 mM sodium azide

species reactivity

human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:300 using protease-idgested, formalin-fixed, paraffin-embedded sections of human or animal tissues
western blot: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
C5992C2931F3418
antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

Gene Information

bovine ... Krt1(100301161)
dog ... Krt1(444857)
human ... KRT1(3848), KRT1(3848), KRT1(3848), KRT5(3852), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856), KRT8(3856)
mouse ... KRT1(16678), KRT1(16678), KRT1(16678), Krt1(16678), Krt5(110308), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691), Krt8(16691)
rat ... Krt1(300250), Krt2-5(369017), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626), Krt2-8(25626)

Gene Information

bovine ... Krt1(100301161)
dog ... Krt1(444857)
human ... KRT1(3848), KRT1(3848), KRT1(3848), KRT5(3852), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856), KRT8(3856)
mouse ... KRT1(16678), KRT1(16678), KRT1(16678), Krt1(16678), Krt5(110308), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691), Krt8(16691)
rat ... Krt1(300250), Krt2-5(369017), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626), Krt2-8(25626)

Gene Information

bovine ... Krt1(100301161)
human ... KRT1(3848), KRT13(3858), KRT13(3858), KRT18(3875), KRT18(3875), KRT4(3851), KRT4(3851), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856)
mouse ... Krt1(16678), Krt10(16661), Krt10(16661), Krt13(16663), Krt13(16663), Krt18(16668), Krt18(16668), Krt4(16682), Krt4(16682), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691)
rat ... Krt1(300250), Krt1-18(706059), Krt1-18(706059), Krt10(450225), Krt10(450225), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626)

Gene Information

bovine ... Krt1(100301161)
human ... KRT1(3848), KRT13(3858), KRT13(3858), KRT18(3875), KRT18(3875), KRT4(3851), KRT4(3851), KRT5(3852), KRT5(3852), KRT6A(3868), KRT6A(3868), KRT6B(3854), KRT6B(3854), KRT8(3856), KRT8(3856)
mouse ... Krt1(16678), Krt10(16661), Krt10(16661), Krt13(16663), Krt13(16663), Krt18(16668), Krt18(16668), Krt4(16682), Krt4(16682), Krt5(110308), Krt5(110308), Krt6a(16687), Krt6a(16687), Krt6b(16688), Krt6b(16688), Krt8(16691), Krt8(16691)
rat ... Krt1(300250), Krt1-18(706059), Krt1-18(706059), Krt10(450225), Krt10(450225), Krt2-5(369017), Krt2-5(369017), Krt2-8(25626), Krt2-8(25626)

species reactivity

human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig

species reactivity

human, chicken, snake, hamster, pig, goat, feline, bovine, carp, rat, rabbit, canine, lizard, sheep, mouse, guinea pig

species reactivity

bovine, mouse, frog, human, kangaroo rat, rat

species reactivity

bovine, mouse, frog, human, kangaroo rat, rat

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

FITC conjugate

clone

PCK-26, monoclonal

clone

PCK-26, monoclonal

clone

C-11, monoclonal

clone

C-11, monoclonal

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Cytokeratin belongs to the member of intermediate filament (IF) protein family.
Cytokeratins, a group comprising at least 29 different proteins are characteristic of epithelial and trichocytic cells. Cytokeratin 1, 5, 6, and 8 are members of the type II neutral-to-basic subfamily. Cytokeratin peptide 1 (68 kDa) is expressed together with cytokeratin 10 in the suprabasal cell layers or the differentiation compartment of the epidermis. Its expression increases with epidermal maturation and it is modified post-translationally in the terminally differentiated keratinocytes of the stratum corneum. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover.

Specificity

The antibody recognizes an epitope located on the Type II cytokeratins 1, 5, 6, and 8. PCK-26 is a broad spectrum antibody which reacts specifically with a variety of normal, reactive, and neoplastic epithelial tissues. The antibody reacts with simple, cornifying, and non-cornifying squamous epithelia and pseudostratified epithelia.

Immunogen

cytokeratin from human epidermis.

Application

Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti-Cytokeratin, pan is a broadly reactive group-type antibody which recognizes an epitope present in most human epithelial tissues. It facilitates the typing of normal, metaplastic and neoplastic cells and it may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micro-metastases in lymph nodes and other tissues and for determining the origin of poorly differentiated tumors.
Monoclonal Anti-Cytokeratin, pan may be used for the localization of cytokeratins using various immunochemical assays such as immunoblotting, dot blotting, and immunohistochemstry (immunofluorescence and immunoenzymatic staining).
A minimum antibody titer of 1:300 was determined by indirect immunofluorescent staining of protease digested, formalin-fixed, paraffin-embedded sections of human or animal tissues.

Biochem/physiol Actions

Pan-cytokeratin along with the expression of CXCR4, may be a prognostic biomarker for non-small cell lung cancer (NSCLC).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Clinical utility of cytokeratins as tumor markers
Barak V, et al.
Clinical Biochemistry, 37(7), 529-540 (2004)
Anup Sood et al.
Viruses, 12(8) (2020-07-29)
Over the last 15 years, advances in immunofluorescence-imaging based cycling methods, antibody conjugation methods, and automated image processing have facilitated the development of a high-resolution, multiplexed tissue immunofluorescence (MxIF) method with single cell-level quantitation termed Cell DIVETM. Originally developed for
Erika M Wall et al.
Cancer research, 67(13), 6442-6450 (2007-07-10)
Immunotherapy of cancer can lead to the selection of antigen loss variants, which provides strong rationale to target oncogenes that are essential for tumor growth or viability. To investigate this concept, we tagged the HER2/neu oncogene with epitopes from ovalbumin
Lei Lv et al.
Cell cycle (Georgetown, Tex.), 11(15), 2864-2875 (2012-07-18)
Most ovarian cancers originate from the ovarian surface epithelium and are characterized by aneuploid karyotypes. Aneuploidy, a consequence of chromosome instability, is an early event during the development of ovarian cancers. However, how aneuploid cells are evolved from normal diploid
Marcus Ruscetti et al.
Cancer research, 75(13), 2749-2759 (2015-05-08)
The epithelial-mesenchymal transition (EMT) has been postulated as a mechanism by which cancer cells acquire the invasive and stem-like traits necessary for distant metastasis. However, direct in vivo evidence for the role of EMT in the formation of cancer stem-like

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service