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C2885

Sigma-Aldrich

CHES

≥99.0% (titration)

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Synonym(s):
2-(Cyclohexylamino)ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H17NO3S
CAS Number:
Molecular Weight:
207.29
Beilstein/REAXYS Number:
2967601
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

assay

≥99.0% (titration)

form

crystalline

pH

3.0-5.0 (103.6 g/L)

useful pH range

8.6-10.0

pKa (25 °C)

9.3

solubility

water: 0.6 g/25 mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OS(=O)(=O)CCNC1CCCCC1

InChI

1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

InChI key

MKWKNSIESPFAQN-UHFFFAOYSA-N

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1 of 4

This Item
C821029311C6013
vibrant-m

C2885

CHES

-
vibrant-m

C8210

CHES

Premium Grade
vibrant-m

29311

CHES

Premium Grade
vibrant-m

C6013

Cholesteryl hemisuccinate tris salt

-
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

pH

3.0-5.0 (103.6 g/L)

pH

3.0-5.0 (0.5 M in H2O)

pH

3.0-5.0 (25 °C, 0.5 M in H2O)

pH

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

solubility

water: 0.6 g/25 mL, clear, colorless

solubility

H2O: 0.5 M, clear, colorless

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

-

form

crystalline

form

powder

form

powder

form

powder

General description

CHES (2-(N-cyclohexylamino)ethanesulphonic acid) is regarded as Good′s buffers for its self-buffering and biocompatible feature. It is used in protein stabilization and does not interact with metals.

Application

CHES has been used:
  • as a buffer in mannanase activity assay of antarctic fungal isolates
  • as a buffer for DNA digestion and labeling of 2′-deoxyadenosine 3′-monophosphate
  • as a component of Wilson and Horne extraction buffer for characterization of rat plasma enzymes

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Hemicellulase activity of antarctic microfungi
Bradner JR, et al.
Journal of Applied Microbiology, 87(3), 366-370 (1999)
Determination of folate in some cereals and commercial cereal-grain products consumed in Poland using trienzyme extraction and high-performance liquid chromatography methods
Gujska E and Kuncewicz A
European Food Research and Technology, 221(1-2), 208-213 (2005)
Good's buffers as a basis for developing self-buffering and biocompatible ionic liquids for biological research
Taha M, et al.
Green Chemistry, 16(6), 3149-3159 (2014)
Detection of Bulky Endogenous Oxidative DNA Lesions Derived from 8, 5?-Cyclo-2?-deoxyadenosine by 32P-Postlabeling Assay
Zhou GD and Moorthy B
Current Protocols in Toxicology / Editorial Board, Mahin D. Maines (editor-in-chief) ... [Et al.], 64(1), 17-17 (2015)
Jun Yang et al.
Langmuir : the ACS journal of surfaces and colloids, 24(18), 10265-10272 (2008-08-20)
A universal nitric oxide (NO) generating surface is assembled via Layer-by-Layer (LbL) deposition of sodium alginate (Alg) and organoselenium modified polyethyleneimine (SePEI) on quartz and polymeric substrates. The immobilized SePEI species is capable of catalytically decomposing S-nitrosothiol species (RSNO) to

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