C3260
Citrate Synthase from porcine heart
ammonium sulfate suspension, ≥100 units/mg protein
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Synonym(s):
Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
biological source
Porcine heart
Quality Level
form
ammonium sulfate suspension
specific activity
≥100 units/mg protein
mol wt
98 kDa ( 49 kDa monomer)
solubility
H2O: soluble 1.0 mg/mL, clear
foreign activity
isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%
storage temp.
2-8°C
Application
Citrate Synthase from porcine heart has been used:
- to inject newt egg for determining its importance in egg activation at fertilization
- in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
- to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs
Biochem/physiol Actions
Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.
Unit Definition
One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.
Physical form
Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.
Preparation Note
Dissolves in water to form a clear solution at 1 mg/mL concentration.
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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