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C3260

Sigma-Aldrich

Citrate Synthase from porcine heart

ammonium sulfate suspension, ≥100 units/mg protein

Synonym(s):

Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)

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About This Item

CAS Number:
9027-96-7
Enzyme Commission number:
4.1.3.7 (BRENDA, IUBMB)
MDL number:
MFCD00130808
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Porcine heart

Quality Level

200

form

ammonium sulfate suspension

specific activity

≥100 units/mg protein

mol wt

98 kDa ( 49 kDa monomer)

solubility

H2O: soluble 1.0 mg/mL, clear

foreign activity

isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%

storage temp.

2-8°C

Application

Citrate Synthase from porcine heart has been used:
  • to inject newt egg for determining its importance in egg activation at fertilization
  • in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
  • to examine whether it can induce a [Ca2+] increase and egg activation in unfertilized eggs

Biochem/physiol Actions

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H2O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Unit Definition

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4 solution, pH 7.0.

Preparation Note

Dissolves in water to form a clear solution at 1 mg/mL concentration.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Instructions for working with enzymes supplied as ammonium sulfate suspensions

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