442610-M

Malate Dehydrogenase, Porcine Heart

Native malate dehydrogenase from porcine heart. Catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD+. Note: 1 KU = 1000 units.

• Synonym(s): Malate Dehydrogenase, Porcine Heart, MDH
• Enzyme Commission number: 1.1.1.37 (BRENDA, IUBMB)
• MDL number: MFCD00131567
• UNSPSC Code: 12352202

Properties

• biological source: Porcine heart
• Quality Level: 100
• form: lyophilized
• specific activity: ≥100 units/mg dry wt.; ≥1000 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• impurities: ≤0.0015% GPT; ≤0.01% GOT; ≤0.01% LDH
• pI: 6.1 - 6.4
• solubility: 0.1 M phosphate buffer: 5 mg/mL
• storage temp.: −20°C

Description

General description

Native malate dehydrogenase from porcine heart. Catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD⁺. Note: 1 KU = 1000 units.

Native malate dehydrogenase from porcine heart. Catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD⁺. Has an optimal pH of 7.2-7.5 and a pI of 6.1-6.4. Note: 1 KU = 1000 units.

Note: 1 KU = 1000 units.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will oxidize 1.0 µmol NADH per min at 25°C, pH 7.5.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable