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C5789

Sigma-Aldrich

Cell Dissociation Solution Non-enzymatic 1x

Prepared in Hanks′ Balanced Salt Solution without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

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MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

product line

BioReagent

form

solution

concentration

1 ×

technique(s)

cell culture | mammalian: suitable

pH

6.5-7.5

shipped in

wet ice

storage temp.

room temp

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C5914T2444C6295
vibrant-m

T2444

Trizma® hydrochloride solution

Premium Grade
concentration

1 ×

concentration

1 ×

concentration

1 M

concentration

-

form

solution

form

liquid

form

solution

form

liquid

pH

6.5-7.5

pH

6.9-7.1

pH

7.6

pH

-

storage temp.

room temp

storage temp.

2-8°C

storage temp.

-

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

dry ice

General description

Cell Dissociation Solution Non-enzymatic buffer systems are alternative to the canonical trypsin based buffers for dissociation of adherent mesenchymal stem cell (MSC) monolayers.

Application

Cell Dissociation Solution Non-enzymatic 1x has been used in the dissociation of murine macrophage (RAW2647) cells, A549 pulmonary cells, islets cells and 2D cell and 3D mammospheres.
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1


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Differential effects of RU486 reveal distinct mechanisms for glucocorticoid repression of prostaglandin E2 release
Chivers JE, et al.
European Journal of Biochemistry, 271(20), 4042-4052 (2004)
Comparison of enzymatic and non-enzymatic means of dissociating adherent monolayers of mesenchymal stem cells
Heng BC, et al.
Biological Procedures Online, 11(1), 161-161 (2009)
Jiaoying Jia et al.
Frontiers in cell and developmental biology, 9, 621187-621187 (2021-08-07)
Dysfunctions of neural stem cells (NSCs) often lead to a variety of neurological diseases. Thus, therapies based on NSCs have gained increasing attention recently. It has been documented that microRNA (miR)-421 represses the autophagy and apoptosis of mouse hippocampal neurons
Rosa G M Lammerts et al.
Frontiers in immunology, 11, 1643-1643 (2020-08-28)
Introduction: Proteinuria contributes to progression of renal damage, partly by complement activation on proximal tubular epithelial cells. By pattern recognition, properdin has shown to bind to heparan sulfate proteoglycans on tubular epithelium and can initiate the alternative complement pathway (AP).
Combinatorial treatment of mammospheres with trastuzumab and salinomycin efficiently targets HER2-positive cancer cells and cancer stem cells
Oak PS, et al.
International Journal of Cancer. Journal International Du Cancer, 131(12), 2808-2819 (2012)

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