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C6243

Sigma-Aldrich

Anti-Cathepsin B antibody, Mouse monoclonal

clone CB59-4B11, purified from hybridoma cell culture

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Synonym(s):
Anti-APPS, Anti-CPSB, Anti-RECEUP

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CB59-4B11, monoclonal

form

buffered aqueous solution

mol wt

antigen ~25 kDa

species reactivity

rat, human

concentration

~1.5 mg/mL

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 4-8 μg/mL using HCT-116 cells.

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CTSB(1508)
rat ... CTSB(64529)

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C4618C8243SAB4200767
clone

CB59-4B11, monoclonal

clone

CPL33/1, monoclonal

clone

CL124-1H6, monoclonal

clone

CTD-19, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

species reactivity

rat, human

species reactivity

human

species reactivity

human

species reactivity

human, rabbit

Gene Information

human ... CTSB(1508)
rat ... CTSB(64529)

Gene Information

human ... CTSL1(1514)

Gene Information

human ... CTSK(1513)

Gene Information

human ... CTSD(1509)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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General description

Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W
The CTSB (Cathepsin B) gene encoding cathepsin B is mapped to human chromosome 8p23.1. Cathepsin B are normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell that take part in local proteolysis. Cathepsin B is both an endo- and exopeptidase, composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor.

Immunogen

Recombinant human cathepsin B.

Application

indirect ELISA, immunohistochemistry, In HCT-116 cells. 4-8μg/mL of antibody concentration may be used. A dilution of 1:800 in human pancreatic carcinoma cell line PANC-1 was used for immunoblotting

Biochem/physiol Actions

Cathepsins are lysosomal proteases activation of enzyme precursors, and tumor invasion and metastasis. It is involved in the proteolysis of many proteins involved in different physiological and pathological pathways. In the brain, this protease, also known as amyloid precursor protein secretase, is responsible for the proteolytic processing of amyloid precursor protein (APP). An incomplete proteolytic processing of APP has been found as a causative factor in Alzheimer disease. Furthermore, ablated genes coding cathepsin B and L leads to enormous neuronal cell death,probably due to autophagy abnormalities. Cathepsin B has been implicated in tumor progression by virtue of localization at the invading front of the tumor. For example, blocking cathepsin B expression in human glioblastoma SNB19 cells by a stable transfection of an expression vector expressing antisense cathepsin B, caused suppression of glioblastoma-induced neovascularization.
Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W.
Cathepsins significantly takes part in the immune response, by processing antigens into peptides for its presentation through MHC class II molecules.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Cysteine Cathepsins and the Cutting Edge of
Cancer Invasion
Gocheva V and Joyce JA
Cell Cycle, 6:1, 60-64 (2007)
Enzymatically active lysosomal proteases are associated with
amyloid deposits in Alzheimer brain
Cataldo AM and Nixon RA
Proceedings of the National Academy of Sciences of the USA, 87, 3861-3865 (1990)
Localization and Activity of Various Lysosomal Proteases in
Leishmania amazonensis-Infected Macrophages
Prina E
Infection and Immunity, 58, 1730-1737 (1990)
Protease signalling in cell death: caspases versus cysteine cathepsins
Turk B and Stoka V
Febs Letters, 581, 2761?2767-2761?2767 (2007)
Blockade of cathepsin B expression in human glioblastoma cells is
associated with suppression of angiogenesis
Yanamandra N
Oncogene, 23, 2224?2230-2224?2230 (2004)

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