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C8541

Sigma-Aldrich

Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse

clone CY-90, ascites fluid

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Synonym(s):
Monoclonal Anti-Cytokeratin Peptide 18
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

CY-90, monoclonal

contains

15 mM sodium azide

species reactivity

wide range

technique(s)

indirect immunofluorescence: 1:800 using formalin-fixed, paraffin-embedded sections of human tissue
western blot: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
C5301C1399C8791
species reactivity

wide range

species reactivity

feline, human, rabbit, canine, bovine

species reactivity

human

species reactivity

human

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

clone

CY-90, monoclonal

clone

M20, monoclonal

clone

KS-B17.2, monoclonal

clone

CKB1, monoclonal

Quality Level

200

Quality Level

200

Quality Level

-

Quality Level

200

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General description

Cytokeratin 18 (45 kDa) is the primary type I keratin expressed in simple epithelial cells. Epithelial cells and their derivatives characteristically contain intermediate filaments (IFs) composed of about 20 related polypeptides with molecular weights between 40,000-69,000. Each epithelial tissue has a specific and stable pattern of expression of some of these cytokeratin subunits. Epithelium derived tumors maintain the expression of the cytokeratins found in the normal tissue of origin. Therefore, carcinomas can be identified and classified by immunocytochemical staining with antibodies that react specifically with cytokeratins.
Monoclonal Anti-Cytokeratin Peptide 18 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Cytokeratin 18 (CK18)/keratin 18 is a structural protein and is present in single-layer epithelia. It is located on human chromosome 12q13.

Specificity

The antibody reacts specifically with a wide variety of simple epithelia (e.g. intestine, respiratory and urinary systems, liver, and glandular epithelia). It does not react with stratified squamous epithelia (e.g. esophagus or epidermis) or with non-epithelial cells.

Immunogen

human epidermal carcinoma A-431 and MCF-7 human breast cancer cell lines.

Application

Monoclonal Anti-Cytokeratin Peptide 18 antibody produced in mouse has been used in:
  • immunofluorescence
  • fluorescence microscopy
  • confocal fluorescence microscopy
  • immunolabelling
  • histoblots

Biochem/physiol Actions

Keratins help to maintain structural integrity. They also control Fas?mediated apoptosis and regulate cell size and protein synthesis. Cytokeratin 18 (CK18) plays a major role in the development of lung cancer. It acts as a therapeutic target for non-small cell lung cancer (NSCLC). CK-18 is considered as a promising plasma biomarker to identify patients with HELLP (hemolysis, elevated liver enzymes, low platelets) syndrome.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Le Ann Blomberg et al.
Molecular reproduction and development, 73(12), 1491-1502 (2006-08-12)
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Role of extracellular signal-regulated kinase pathway in RRR-alpha-tocopheryl succinate-induced differentiation of human MDA-MB-435 breast cancer cells
You H, et al.
Molecular Carcinogenesis, 33(4), 228-236 (2002)
Matthew J Lawson et al.
Royal Society open science, 8(11), 211067-211067 (2021-11-06)
Micro-computed tomography (µCT) provides non-destructive three-dimensional (3D) imaging of soft tissue microstructures. Specific features in µCT images can be identified using correlated two-dimensional (2D) histology images allowing manual segmentation. However, this is very time-consuming and requires specialist knowledge of the
Thomas Brabletz et al.
Cancer research, 64(19), 6973-6977 (2004-10-07)
The homeobox transcription factor Cdx2 specifies intestinal development and homeostasis and is considered a tumor suppressor in colorectal carcinogenesis. However, Cdx2 mutations are rarely found. Invasion of colorectal cancer is characterized by a transient loss of differentiation and nuclear accumulation

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