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AB1 cell line from mouse lung

10092305

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biological source

mouse lung

description

Mouse malignant mesothelioma

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast-like but shows a typical epithelial "cobblestone"

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse malignant mesothelioma

Cell Line Description

Mice (BALB/c female 6-8 weeks) were exposed to crocidolite asbestos through intraperitoneal injection resulting in tumour development. Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic immunocompetent mice. Cell Bank Australia reference number: CBA0144

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Culture Medium

RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 5% FCS

Subculture Routine

Optimal split ratio 1:8 (seeding density 1.2 x104 cells/cm2).Cells incubated at 37oC with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake, University of Western Australia, Australia

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