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VP002

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VirusExpress® 293 AAV Production Cells

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Synonym(s):
HEK293 cells for AAV production

Quality Level

600

shipped in

liquid nitrogen

storage temp.

−196°C

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VP00114385C72181
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VP002

VirusExpress® 293 AAV Production Cells

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SAFC

VP001

VirusExpress® 293T Lentiviral Production Cells

EX-CELL® CD HEK293 Viral Vector Medium Chemically defined, animal component-free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

SAFC

14385C

EX-CELL® CD HEK293 Viral Vector Medium

293-Free Transfection Reagent Animal-free polycationic liposomal transfection reagent optimized for the transfection of HEK293 cells grown in suspension culture.

Sigma-Aldrich

72181

293-Free Transfection Reagent

storage temp.

−196°C

storage temp.

−196°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

600

Quality Level

600

Quality Level

500

Quality Level

200

General description

VirusExpress® 293 AAV Production Cells are a clonal, suspension-adapted HEK293 derivative selected for fast growth, high transfection efficiency, and high-titer AAV production. VirusExpress 293 AAV Production Cells grow to high cell densities and are amenable to chemical-based transfection reagents commonly used in manufacturing (i.e., PEI, lipid-nanoparticle). A medium exchange step is not needed prior to transfection, and the cells can be transfected in larger volumes, enabling scale-up of AAV production. The cells are supplied in a chemically defined media formulation and can be thawed directly into EX-CELL® CD HEK293 Viral Vector Medium.

VirusExpress 293 AAV Production Cells are derived from the HEK293 cell line (ATCC CRL-1573) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham F.L. et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs.

Packaging

This product is shipped in a liquid nitrogen dry vapor shipper. The liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt.

Other Notes

The cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL. The cells were banked under cGMP conditions in chemically defined medium and 8% DMSO.

Legal Information

A commercial license must be taken prior to use of the cells, or any products or materials derived from the cells, in a manufacturing process. Please contact your local sales representative for more details.
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Articles

Scalability Challenges of Viral Vector Manufacturing

Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.

Bioreactor Process Development and Scale-Up for Transient Transfection-Based Lentivirus Manufacturing in Suspension Cell Culture

A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.

Bioprocessing And Scale-Up Of Suspension HEK293 Cells For AAV Manufacturing

To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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