All Photos(1)



VirusExpress® 293 AAV Production Cells

HEK293 cells for AAV production

Quality Level

shipped in

liquid nitrogen

storage temp.


Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
storage temp.


storage temp.


storage temp.


storage temp.


Quality Level


Quality Level


Quality Level


Quality Level


General description

VirusExpress® 293 AAV Production Cells are a clonal, suspension-adapted HEK293 derivative selected for fast growth, high transfection efficiency, and high-titer AAV production. VirusExpress 293 AAV Production Cells grow to high cell densities and are amenable to chemical-based transfection reagents commonly used in manufacturing (i.e., PEI, lipid-nanoparticle). A medium exchange step is not needed prior to transfection, and the cells can be transfected in larger volumes, enabling scale-up of AAV production. The cells are supplied in a chemically defined media formulation and can be thawed directly into EX-CELL® CD HEK293 Viral Vector Medium.

VirusExpress 293 AAV Production Cells are derived from the HEK293 cell line (ATCC CRL-1573) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham F.L. et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs.


This product is shipped in a liquid nitrogen dry vapor shipper. The liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt.

Other Notes

The cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL. The cells were banked under cGMP conditions in chemically defined medium and 8% DMSO.

Legal Information

A commercial license must be taken prior to use of the cells, or any products or materials derived from the cells, in a manufacturing process. Please contact your local sales representative for more details.
EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC
VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA


Scalability Challenges of Viral Vector Manufacturing

Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.

Bioreactor Process Development and Scale-Up for Transient Transfection-Based Lentivirus Manufacturing in Suspension Cell Culture

A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.

Bioprocessing And Scale-Up Of Suspension HEK293 Cells For AAV Manufacturing

To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service