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VirusExpress® 293T Lentiviral Production Cells

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Synonym(s):
HEK293T cells for lentivirus production

shipped in

liquid nitrogen

Quality Level

storage temp.

−196°C

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CHOZN® CHO K1 Host Cell Line Suspension-adapted in CD media

SAFC

CHOK1

CHOZN® CHO K1 Host Cell Line

storage temp.

−196°C

storage temp.

2-8°C

storage temp.

−196°C

storage temp.

-

Quality Level

600

Quality Level

200

Quality Level

200

Quality Level

100

General description

VirusExpress 293T Lentiviral Production Cells are a clonal, suspension-adapted HEK293T derivative selected for fast growth, high transfection efficiency, and high-titer lentivirus production. VirusExpress 293T Lentiviral Production Cells grow to high cell densities, exhibit minimal cell clumping, and are amenable to PEI-based transfection. A medium exchange step is not needed prior to transfection with a PEI transfection reagent, and the cells can be transfected in larger volumes, enabling scale-up of lentivirus production. The cells are intended to be cultured in, and can be directly thawed into EX-CELL® CD HEK293 Viral Vector Medium.

VirusExpress 293T Lentiviral Production Cells are derived from the HEK293T cell line (ATCC CRL-3216) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham FL et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. HEK293T cells were created by stable transfection of the HEK293 cell line with a temperature-sensitive mutant of SV40 large T antigen. Due to the expression of the large T antigen, transfected plasmids that carry the SV40 origin of replication can replicate in 293T cells and will transiently maintain a high copy number. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs.

Packaging

This product is shipped in a liquid nitrogen dry vapor shipper. The liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt.

Other Notes

The cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL. The cells were banked using under cGMP conditions in chemically defined medium supplement with 6 mM glutamine and 8% DMSO.

Legal Information

A commercial license must be taken prior to use of the cells, or any products or materials derived from the cells, in a manufacturing process. Please contact your local sales representative for more details.
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.

A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.

To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.

Optimizing the upstream portion of gene therapy production sets the stage for successful manufacturing by maximizing viral vector titers. To increase upstream titers and process productivity, manufacturers must partner with upstream technology experts who work with HEK293, HEK293T and Sf9 cells. Draw on our experience today to speed your gene therapy to patients.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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