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VP001
VirusExpress® 293T Lentiviral Production Cells
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This Item | VP002 | 14385C | EC896 |
|---|---|---|---|
| storage temp. −196°C | storage temp. −196°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 600 | Quality Level 600 | Quality Level 500 | Quality Level 100 |
General description
VirusExpress 293T Lentiviral Production Cells are derived from the HEK293T cell line (ATCC CRL-3216) through single cell cloning. The parental HEK293 cell line was established by transformation of human embryonic kidney cells with sheared human adenovirus 5 DNA (Graham FL et al. 1977). A 4-kb adenoviral genome fragment is known to have integrated into chromosome 19 and encodes for E1A/E1B proteins. HEK293T cells were created by stable transfection of the HEK293 cell line with a temperature-sensitive mutant of SV40 large T antigen. Due to the expression of the large T antigen, transfected plasmids that carry the SV40 origin of replication can replicate in 293T cells and will transiently maintain a high copy number. Our clonal derivative eliminates variability from the population while also being adapted to suspension, serum-free conditions. Suspension culture and chemically defined medium allow scalability into stirred tank bioreactors for yields that can meet commercial needs.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Articles
Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.
A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.
To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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