MilliporeSigma
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14571C

SAFC

EX-CELL® 293 Serum-Free Medium for HEK 293 Cells

Serum-free, Animal-protein free, without L-glutamine, liquid, suitable for cell culture

MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile; sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

NaHCO3: yes
HEPES: no
L-glutamine: no
glucose: yes

shipped in

ambient

storage temp.

2-8°C

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This Item
24571C14660C14581C
form

liquid

form

dry powder

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: yes
HEPES: no
L-glutamine: no
glucose: yes

components

-

components

L-glutamine: no
glucose: 4.0 g/L
HEPES: 7.5 mM
NaHCO3: 1.05 g/L

components

glucose: 6.0 g/L
NaHCO3: 1.8 g/L
HEPES: no
L-glutamine: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

EX-CELL 293 is an animal-protein free, serum-free medium developed for the long-term growth of Human Embryonic Kidney 293 (HEK 293) and related cells. The cells, in a suspension culture, can be subcultured directly into EX-CELL 293 from serum-supplemented media with little or no adaptation. Suspension cultures can be maintained, without refeeding, for about 10 days and can be carried for more that 20 passages with no loss of viability.
EX-CELL 293 is an animal-protein free, serum-free medium developed for the long-term growth of Human Embryonic Kidney 293 (HEK 293) and related cells. The cells, in a suspension culture, can be subcultured directly into EX-CELL 293 from serum-supplemented media with little or no adaptation. Suspension cultures can be maintained, without refeeding, for about 10 days and can be carried for more that 20 passages with no loss of viability.

Legal Information

EX-CELL is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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