MilliporeSigma
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1.02376

SAFC

Cellvento® BHK-200

serum-free, ≤10 IU/mL endotoxin

All Photos(1)

Quality Level

500

form

dry powder

technique(s)

cell culture | mammalian: suitable (suspension)

shipped in

ambient

storage temp.

2-8°C

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This Item
1.024851.024881.02482
Cellvento® BHK-200 serum-free, ≤10 IU/mL endotoxin

SAFC

1.02376

Cellvento® BHK-200

Cellvento® CHO-210 ≤1.0 IU/mL endotoxin, pH 3.9-4.5(without supplement)

SAFC

1.02485

Cellvento® CHO-210

Cellvento® Feed-210 ≤1.0 IU/mL endotoxin, pH 5.2-5.8

SAFC

1.02488

Cellvento® Feed-210

Cellvento® CHO-110 Chemically defined cell culture medium

SAFC

1.02482

Cellvento® CHO-110

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

impurities

≤10 IU/mL endotoxin

impurities

≤1.0 IU/mL endotoxin

impurities

≤1.0 IU/mL endotoxin

impurities

-

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

500

General description

Cellvento® BHK-200 medium is a serum-free cell culture medium, formulated without any animal derived component and optimized for the culture of suspension BHK21 cells at high-density and viability and efficient propagation of viruses. BHK21 cells can be grown as suspension cultures in T-flasks, shaker flasks, spinner bottles or stirred tank bioreactors. Cellvento® BHK-200 medium has been developed for BHK-21 cell line but is not limited to this application and may suit other vaccine producing cell lines as well.

Application

Cellvento® BHK-200 medium has been shown to be an optimal formulation for the growth and maintenance of BHK21 suspension cell lines used for viral vaccine production, and qualified for instance for the production of Foot and Mouth Disease virus (FMDV). When the density of BHK21 cells satisfies process requirement, the virus can be inoculated directly to the reactor without media exchange or cell separation/ sedimentation steps, resulting in highly efficient virus production. It is recommended to use only 70% of the maximum bioreactor volume for cell growth. After cells have reached the desired cell density, 30% fresh medium can be added in the reactor before inoculation and propagation of the virus.

Quantity

Formulated to contain 21.6658 grams of powder per liter of medium.

Legal Information

CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

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