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14610C

SAFC

EX-CELL® 610-HSF Serum-Free Medium for Hybridoma Cells

Low-protein, with L-glutamine, liquid, sterile-filtered, suitable for cell culture

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MDL number:
NACRES:
NA.75

description

Drug Master File available
for research or for further manufacturing use

Quality Level

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

components

glucose: 3.45 g/L
NaHCO3: 1.60 g/L
L-glutamine: 0.333 g/L
HEPES: 7.5 mM

shipped in

ambient

storage temp.

2-8°C

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This Item
14621C14660CH4409
form

liquid

form

liquid

form

liquid

form

liquid

storage condition

protect from light

storage condition

-

storage condition

-

storage condition

-

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

components

glucose: 3.45 g/L
NaHCO3: 1.60 g/L
L-glutamine: 0.333 g/L
HEPES: 7.5 mM

components

NaHCO3: 1.6 g/L
HEPES: 7.5 mM
L-glutamine: no
glucose: 3.45 g/L

components

L-glutamine: no
glucose: 4.0 g/L
HEPES: 7.5 mM
NaHCO3: 1.05 g/L

components

glucose: 2.84 g/L
NaHCO3: yes
L-glutamine: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Application

EX-CELL 610-HSF is a low-protein (11 mg/L) serum-free medium. Originally developed to support the growth of hybrid cells in culture, it has been shown to support a wide range of cells including lymphoid and epithelial cells and B cell hybridomas of murine, rat and human origin. EX-CELL 610-HSF has been used in stationary culture systems and in large-scale bioreactors. In both types of culture, the production of cellular products, particularly monoclonal antibodies, has been shown to equal or exceed levels seen when the same cells are cultured in the presence of Fetal Bovine Serum (FBS).

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Gunasekaran Dhandapani et al.
mAbs, 11(3), 583-592 (2019-01-09)
We introduce a new concept and potentially general platform for antibody (Ab) purification that does not rely on chromatography or specific ligands (e.g., Protein A); rather, it makes use of detergent aggregates capable of efficiently capturing Ab while rejecting hydrophilic

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