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HeB5

11033104, mouse liver, Epithelial

Synonym(s):

Hepa-1c1c7

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About This Item

UNSPSC Code:
41106514

product name

HeB5, 11033104

biological source

mouse liver

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

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Related Categories

Cell Line Origin

Hepa-1c1c7, genetic modification, Xenobiotic-Responsive Element (XRE)

Cell Line Description

HeB5 is a derivative of Hepa-1c1c7 (Sigma product number 95090613) cell line stably transfected with a firefly luciferase reporter construct bearing Xenobiotic-Responsive Element (XRE) driven by a TATA promoter. HeB5 can be used for detection of mouse CYP1A induction by chemicals.

Culture Medium

Alpha-MEM + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16μg/ml Blasticidin S HCL.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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