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product name
S1814.PB5, 85011402
biological source
human embryo
growth mode
Adherent
karyotype
Diploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human embryo fibroblast
Cell Line Description
Human embryo fibroblast cell line.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9
TPOX: 9,11
vWA: 15
CSF1PO: 10,12
D13S317: 10,12
D16S539: 11
D5S818: 13
D7S820: 10,11
THO1: 7,9
TPOX: 9,11
vWA: 15
Culture Medium
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells should also grow in DMEM or RPMI 1640. This cell line also grow well in HAT.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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