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product name
BRL 3A, 85111503
biological source
rat liver
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
MSA factors
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Buffalo Rat liver
Cell Line Description
Originally isolated by primary cloning. Serum-free medium conditioned by the cells produces a family of polypeptides termed multiplication stimulating activity (MSA).
Application
Growth factor studies
Culture Medium
Coon′s modified Ham′s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS) or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 5% Foetal Bovine Serum (FBS) + 2mM Glutamine.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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