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product name
L52, 91012321
biological source
Porcine blood
growth mode
Suspension
karyotype
average No 36.5
morphology
Not specified
products
Immunoglobulin M (IgM)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Porcine peripheral blood B cell
Cell Line Description
One of a series of cell lines established from mononuclear cells of a boar (Sus scrofa domestica L) which was injected with Shimozuma cells producing porcine retrovirus (Tsukuba-1). The cells contain retrovirus particles and are positive in RT assay.
DNA Profile
Not specified
Culture Medium
RPMI 1640 + 2 mM Glutamine + 1 mM Sodium Pyruvate (NaP) + 0.05 mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO2; 37 °C. Cells grow as small clumps.
Other Notes
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Certificates of Analysis (COA)
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