biological source
human kidney
packaging
tube of 5 μg 93120819-DNA-5UG
pkg of vial of cells 93120819-1VL
growth mode
Adherent
karyotype
Hypentaploid to hypohexaploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Derived from a 69 year old Caucasian male with a kidney carcinoma. The cells contain microfilaments and multilaminar bodies. They also exhibit microvilli. The cells are tumourigenic in nude mice.
Human Caucasian kidney carcinoma
Preparation Note
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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