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RPMI 8866 Cell Line human

95041316, Lymphoblastoid from blood

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Synonym(s):
R8866 Cells, RPMI8866 Cells, Roswell Park Memorial Institute 8866 Cells

biological source

human blood

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

products

Not specified

receptors

Immunoglobulin E (IgE) binding factor, Calcitonin

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human B lymphocyte

Cell Line Description

RPMI 8866 has been established from the peripheral blood of a 51-year-old American woman with chronic myelogenous leukaemia in May 1966. The B lymphoid cell line expresses a relatively high amount of calcitonin receptors, membrane bound Ig and soluble IgE-binding factor (Fc epsilon RII/CD23).

Application

Receptor studies, binding assays, hormone studies.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10,14
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 9.3
TPOX: 8,11
vWA: 14,16

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

To keep the cells in exponential growth maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. If starting from a frozen ampoule, add thawed cells to a conical based centrifuge tube e.g. 15ml tube. Slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 200ul, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 10% serum. Incubate flask at 37 C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth, this can take up to 7 days.

Other Notes

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