RPMI 8226

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012702, human blood, Not specified

• Synonym(s): RPMI8226 Cells, Roswell Park Memorial Institute 8226 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human blood
• packaging: tube of 5 μg 87012702-DNA-5UG; pkg of vial of cells 87012702-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Not specified
• products: Immunoglobulin G (Ig) lambda light chain
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

RPMI 8226 has been used:

• to grow human myeloma cell lines
• to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
• to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line

Biochem/physiol Actions

Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.

Human myeloma

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

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