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RPMI 8226


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RPMI8226 Cells, Roswell Park Memorial Institute 8226 Cells

biological source

human blood

growth mode



Not specified


Not specified


Immunoglobulin G (Ig) lambda light chain


Not specified


cell culture | mammalian: suitable

relevant disease(s)


shipped in

dry ice

storage temp.


Related Categories

Cell Line Origin

Human myeloma

Cell Line Description

Derived from the peripheral blood of a 61 year old male with multiple myeloma. The cells produce and secrete Ig lambda light chain.


RPMI 8226 has been used:
  • to grow human myeloma cell lines
  • to evaluate the effect of 1-aryl-2-methylthioimidazolines (IIV) on the viability of RPMI 8226 cells
  • to measure cytokine production by bone marrow stromal cells (BMSCs) after contact with the RPMI 8226 cell line

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11
D16S539: 9
D5S818: 11,13
D7S820: 9,10
THO1: 8
TPOX: 8,11
vWA: 16,18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. After resuscitation cell debris will be present in culture. This will decrease with subsequent subculture.

Other Notes

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