b.End5
96091930, mouse brain, Endothelial-like
Synonym(s):
Brain-derived Endothelial 5 Cells, bEnd3 Cells, bEnd5 Cells
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About This Item
UNSPSC Code:
41106514
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product name
b.End5, 96091930
biological source
mouse brain
growth mode
Adherent
karyotype
Not specified
morphology
Endothelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse Balb/c brain endothelioma
Cell Line Description
The cell line b.End5 has been established from brain endothelial cells of BALB/c mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End5 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as CAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.
Application
Study of endothelial cell growth, differentiation and influence of oncogenes.
Culture Medium
DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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