All Photos(1)




Enzymatic Protein Biotinylation Kit

for biotinylation of up to 20 mg target protein

Sign Into View Organizational & Contract Pricing

Quality Level

shipped in

dry ice

storage temp.


Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
BiotinTag™ Micro Biotinylation Kit


BiotinTag Micro Biotinylation Kit

ImmunoProbe™ Biotinylation Kit


ImmunoProbe Biotinylation Kit

storage temp.


storage temp.


storage temp.


storage temp.


Quality Level


Quality Level


Quality Level


Quality Level


General description

Biotinylated proteins are used in a variety of applications, such as binding assays, biopanning, and protein purification. Although chemical biotinylation methods are common, they present several challenges, including partial labeling and high batch to-batch variation, because of the random nature of these methods. In addition, the lack of control of the biotinylation site might affect the biotinylated protein′s activity. The Enzymatic Protein Biotinylation Kit allows direct and specific biotinylation using a specific biotin ligation enzyme. The kit contains all the components required for in vitro biotinylation of up to 20 mg recombinant target protein. Biotinylation is mediated by the enzyme BirA biotin ligase, which uniformly ligates biotin to lysine residues in the context of several tags, such as AviTag and Biotag. This kit was tested successfully under a wide range of conditions, including:
  • Temperatures ranging from 4 °C to 30 °C
  • pH from 6.0 to 9.0
  • NaCl concentrations from 50 to 500 mM.
The kit includes streptavidin, which can be used to assess the degree of biotinylation. The kit also includes a BLPR-tagged protein as a positive control.
Supportive calculator (Click here to download a calculator excel file): Will calculate the reagents needed according to your experimental needs.

Legal Information

AviTag is a trademark of Avidity, LLC

Storage Class

10 - Combustible liquids



Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service