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11418165001

Roche

Biotin Protein Labeling Kit

sufficient for 5 labeling reactions, kit of 1 (5 components), suitable for immunoblotting, suitable for ELISA

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Synonym(s):
Biotin, protein labeling

usage

sufficient for 5 labeling reactions

Quality Level

packaging

kit of 1 (5 components)

manufacturer/tradename

Roche

technique(s)

ELISA: suitable
immunoblotting: suitable

storage temp.

2-8°C

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manufacturer/tradename

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manufacturer/tradename

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manufacturer/tradename

Roche

manufacturer/tradename

Roche

technique(s)

ELISA: suitable, immunoblotting: suitable

technique(s)

capture ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, indirect ELISA: 1:4,000

technique(s)

FISH: suitable

technique(s)

Northern blotting: suitable, Southern blotting: suitable, hybridization: suitable

packaging

kit of 1 (5 components)

packaging

-

packaging

kit of 1 (7 components)

packaging

kit of 1 (12 components)

usage

sufficient for 5 labeling reactions

usage

-

usage

sufficient for 50 labeling reactions

usage

sufficient for 2 x 10 labeling reactions

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

For labeling of proteins with biotin.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.

Application

Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.

Packaging

1 kit containing 5 components.

Quality

Function tested.

Preparation Note

Working solution: Preparation of the solutions
  • Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
  • PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
  • Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • Blocking Reagent (green screw cap)

  • Phosphate Buffered Saline (PBS) (blue screw cap)

  • D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)

  • Dimethylsulfoxide (DMSO) (red screw cap)

  • Sephadex G-25 Columns

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1


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