CS0340
Dihydrofolate Reductase Assay Kit
1 kit sufficient for 50-100 tests
Recommended Products
Quality Level
usage
kit sufficient for 50-100 tests
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... DHFR(1719)
Related Categories
Application
Dihydrofolic acid+NADPH+H+ ↔ Tetrahydrofolic acid+NADP+
Biochem/physiol Actions
Features and Benefits
- Quick and simple method.
- The kit contains all the reagents required for a colorimetric assay of DHFR activity in cell lysates, tissue homogenates, or column fractions of purified enzyme.
- The kit includes a purified enzyme for use as a positive control and screening of DHFR inhibitors.
- The kit includes methotrexate (MTX), a prokaryotic and eukaryotic DHFR specific inhibitor, which exhibits anti-tumor activities.
- The kit was tested on A431, NIH-3T3, and CHO cell lines, rat liver, kidney, brain, and skeletal muscle tissue extracts, and recombinant DHFR.
Kit Components Only
- Assay Buffer 10x for DHFR 30 mL
- Dihydrofolate Reductase (DHFR) human .1 U
- Dihydrofolic acid (DHFR substrate) 3 x 10
- Amethopterin (+)(methotrexate, MTX)
(DHFR inhibitor) 2 x 10 - NADPH (β-Nicotinamide adenine dinucleotide phosphate reduced tetrasodium salt) 25 mg
signalword
Danger
Hazard Classifications
Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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