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CS0980

Sigma-Aldrich

Chitinase Assay Kit

sufficient for 100 multiwell tests

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usage

sufficient for 100 multiwell tests

Quality Level

absorbance ratio

405 nm (Absorbance)

shipped in

wet ice

storage temp.

2-8°C

Gene Information

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Chitinase Assay Kit sufficient for 100 multiwell tests

CS0980

Chitinase Assay Kit

Chitinase Assay Kit, Fluorimetric sufficient for 200 multiwell tests

CS1030

Chitinase Assay Kit, Fluorimetric

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CS0260

Glutathione Assay Kit

Citrate Synthase Assay Kit 1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

CS0720

Citrate Synthase Assay Kit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

usage

sufficient for 100 multiwell tests

usage

sufficient for 200 multiwell tests

usage

sufficient for 700 assays

usage

 kit sufficient for 100 reactions (using a 1 ml cuvette),  kit sufficient for 480 reactions (using 96 multiwell plates)

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

absorbance ratio

405 nm (Absorbance)

absorbance ratio

-

absorbance ratio

-

absorbance ratio

-

General description

The Chitinase Assay Kit is based on the enzymatic hydrolysis of chitinase substrates. The hydrolysis releases p-nitrophenol, which can be measured colormetrically at 405 nm.

Application

Chitinase Assay Kit has been used in screening chitinase activity of recombinant chitinase from yeast, Metschnikowia fructicola, bacterial endophyte 3A12 and Clostridium difficile spores.

The kit provides all the reagents required for efficient detection of chitinase activity in fungal and bacterial growth media, macrophage lysates, and purified enzyme preparations. In addition, the kit provides three different substrates for the detection of the various types of the chitinolytic activity:
  • 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside - substrate suitable for exochitinase activity detection (chitobiosidase activity)
  • 4-Nitrophenyl N-acetyl-β-D-glucosaminide - substrate suitable for exochitinase activity detection (β-N-acetylglucosaminidase activity)
4-Nitrophenyl β-D-N,N′,N′′-triacetylchitotriose - substrate suitable for endochitinase activity detection

Biochem/physiol Actions

Chitinase catalyzes the hydrolytic cleavage of the β-1→4-glycoside bond present in biopolymers of N-acetylglucosamine, primarily in chitin. Chitinases are widely distributed in living organisms and are found in fungi, bacteria, parasites, plants, and animals. They are classified in families based on amino acid sequence similarities.

The chitinolytic enzymes are also categorized based on their enzymatic action on chitin substrates. Endochitinases are defined as the enzymes catalyzing the random cleavage at internal points in the chitin chain. Exochitinases catalyze the progressive release of acetylchitobiose or N-acetylglucosamine from the non-reducing end of chitin, and are referred to as chitobiosidase and β-N-acetylglucosaminidase, respectively.

Chitinases perform different functions in different organisms. In bacteria, they are mainly involved in nutritional processes. In yeast and various fungi, these enzymes participate in morphogenesis. In animals and plants, chitinases primarily play a role in the defense of the organism against pathogen attack.

Analysis Note

The kit assay is based on the enzymatic hydrolysis of chitinase substrates. This hydrolysis releases p-nitrophenol (4-nitrophenol), which upon ionization in basic pH can be measured colorimetrically at 405 nm.

Kit Components Only

Product No.
Description

  • Assay Buffer 20 mL

  • 4-Nitrophenyl N-acetyl-β-D-glucosaminide 10 mg

  • 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside 5 mg

  • 4-Nitrophenyl β-D-N,N′N′′-triacetylchitotriose 1 mg

  • Chitinase from Trichoderma viride 1 mg

  • p-Nitrophenol Solution, 10 mM 1 mL

  • Sodium Carbonate 1 g

related product

Product No.
Description
Pricing

pictograms

Health hazard

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Isolation, purification, crystallization and preliminary crystallographic studies of a chitinase from Crocus vernus
Akrem,A et al.
Acta Crystallographica Section A, Foundations of Crystallography, F67, 340-343 (2011)
Hanan R Shehata et al.
Frontiers in microbiology, 7, 1548-1548 (2016-10-21)
Endophytes are microbes that inhabit internal plant tissues without causing disease. Some endophytes are known to combat pathogens. The corn (maize) landrace Chapalote has been grown continuously by subsistence farmers in the Americas since 1000 BC, without the use of
Önder Kimyon et al.
Frontiers in microbiology, 7, 1313-1313 (2016-09-08)
N-acetyl glucosamine, the monomer of chitin, is an abundant source of carbon and nitrogen in nature as it is the main component and breakdown product of many structural polymers. Some bacteria use N-acyl-L-homoserine lactone (AHL) mediated quorum sensing (QS) to
Grace Chong et al.
Microbial biotechnology, 5(5), 621-633 (2012-05-16)
Activated sludge used for wastewater treatment globally is composed of a high-density microbial community of great biotechnological significance. In this study the presence and purpose of quorum sensing via N-acylated-l-homoserine lactones (AHLs) in activated sludge was explored. The presence of
Flávio Augusto Cardozo et al.
World journal of microbiology & biotechnology, 35(8), 114-114 (2019-07-25)
N-acetyl-D-glucosamine (GlcNAc) is an important amino-monosaccharide with great potential for biotechnological applications. It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs. Therefore, enzymatic production

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