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CS1030

Sigma-Aldrich

Chitinase Assay Kit, Fluorimetric

sufficient for 200 multiwell tests

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NACRES:
NA.84

usage

sufficient for 200 multiwell tests

Quality Level

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CHIT1(1118)

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This Item
CS0980CS1020CASP3F
Chitinase Assay Kit, Fluorimetric sufficient for 200 multiwell tests

CS1030

Chitinase Assay Kit, Fluorimetric

Chitinase Assay Kit sufficient for 100 multiwell tests

CS0980

Chitinase Assay Kit

Caspase 3 Assay Kit, Fluorimetric

CASP3F

Caspase 3 Assay Kit, Fluorimetric

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

sufficient for 200 multiwell tests

usage

sufficient for 100 multiwell tests

usage

sufficient for 200 multiwell tests

usage

sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml)

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

General description

The kit assay is based on the enzymatic hydrolysis of chitinase substrates. This enzymatic hydrolysis releases 4-methylumbelliferone (4MU), which upon ionization in basic pH, can be measured fluorimetrically at an excitation wavelength of 360 nm and an emission wavelength of 450 nm. The use of fluorimetric substrates provides a very sensitive detection system.

Application

The Chitinase Assay Kit provides all the reagents required for efficient and sensitive detection of chitinase activity in fungal and bacterial growth media, macrophage lysates, and purified enzyme preparations. In addition, the kit provides three different substrates for the detection of the various types of the chitinolytic activity:
  • 4-Methylumbelliferyl N,N′-diacetyl-β-D-chitobioside - substrate suitable for exochitinase activity detection (chitobiosidase activity)
  • 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide - substrate suitable for exochitinase activity detection (β-N-acetylglucosaminidase activity)
  • 4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotriose - substrate suitable for endochitinase activity detection

Biochem/physiol Actions

Chitinase catalyzes the hydrolytic cleavage of the β-1→4-glycoside bond present in biopolymers of N-acetylglucosamine, primarily in chitin. Chitinases are widely distributed in living organisms and are found in fungi, bacteria, parasites, plants, and animals. They are classified in families based on amino acid sequence similarities.
The chitinolytic enzymes are also categorized based on their enzymatic action on chitin substrates. Endochitinases are defined as the enzymes catalyzing the random cleavage at internal points in the chitin chain. Exochitinases catalyze the progressive release of acetylchitobiose or N-acetylglucosamine from the non-reducing end of chitin, and are referred to as chitobiosidase and β-N-acetylglucosaminidase, respectively.
Chitinases perform different functions in different organisms. In bacteria, they are mainly involved in nutritional processes. In yeast and various fungi, these enzymes participate in morphogenesis. In animals and plants, chitinases primarily play a role in the defense of the organism against pathogen attack.

Suitability

The kit was tested and found suitable for Trichoderma viride and Streptomyces griseus, along with Hela, Jurkat, CHO, NIH-3T3, U-837 mammalian cell lines, human macropages, rat lung, kidney, liver, and brain tissue.

Preparation Note

Use ultrapure water for preparation of reagents.

Kit Components Only

Product No.
Description

  • Assay Buffer 25 mL

  • 4-Methylumbelliferyl N-acetyl-β-D-glucosaminide 5 mg

  • 4-Methylumbelliferyl β-D-N,N′-diacetylchitobioside hydrate 5 mg

  • 4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotriose 5 mg

  • Chitinase from Trichoderma viride 1 mg

  • 4-Methylumbelliferone Standard Solution, 50 mg/mL 1 mL

  • Sodium Carbonate 2 g

  • Dimethyl Sulfoxide 1 mL

See All (8)

pictograms

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Hazard Classifications

Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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