CTI1
CompoZr® Targeted Integration Kit - AAVS1
Transgene Integration in the Human Genome
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General description
Features and Benefits
The ZFNs engineered to target AAVS1 are a well-validated pair that cut the DNA with high efficiency. ZFN-aided targeted integration can achieve 20% efficiency in some cell types without antibiotic selection, representing an improvement of three to four orders of magnitude in efficiency over normal homologous recombination events1.
•Rapid biallelic insertions through a single transfection
ZFN mediated homology-directed repair leads to gene addition within 48 hours of transfection of ZFN mRNA/plasmid donor, with both monoallelic and biallelic insertions1.
•Controlled transgene effects through use of single genomic locus
In the past, most transgenic cell lines have been constructed via random integration of a plasmid. This results in a collection of clones with greatly varied expression levels and expression stability. Targeted integration into the AAVS1 locus will provide a common integration site with stable, uniform expression levels1.
• Eliminates need to engineer cell lines with transgene landing pads
Transgene integration into the genome has previously been achieved via recombination through recombinases such as Cre and FLP. This requires prior insertion of a transgene landing pad into the genome through random integration followed by transgene addition, making it a lengthy two step process. Targeted integration into AAVS1 eliminates the first step, making transgene expression a simple 1-step process1.
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10 - Combustible liquids
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