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D219

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Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse

clone 20A, ascites fluid, buffered aqueous solution

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Synonym(s):
Anti-CACNA2, Anti-CACNL2A, Anti-CCHL2A, Anti-LINC01112, Anti-lncRNA-N3
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

20A, monoclonal

form

buffered aqueous solution

mol wt

antigen 143 kDa (reduced)
antigen 220 kDa (non-reduced)

species reactivity

rat, mouse, rabbit, guinea pig

technique(s)

immunohistochemistry (frozen sections): 1:500
western blot: 1:500

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

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conjugate

unconjugated

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unconjugated

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

mouse ... Cacna2d1(12293)
rat ... Cacna2d1(25399)

Gene Information

human ... ATP1A3(478)
rat ... Atp1a3(24213)

Gene Information

human ... RYR1(6261), RYR2(6262)
mouse ... Ryr1(20190), Ryr2(20191)
rat ... Ryr1(114207), Ryr2(84025)

Gene Information

human ... ACTN2(88)
mouse ... Actn2(11472)
rat ... Actn2(291245)

species reactivity

rat, mouse, rabbit, guinea pig

species reactivity

human, rat, monkey, sheep, rabbit, guinea pig, canine

species reactivity

human, rat, rabbit, canine, mink, bovine, frog, fish, primate, chicken, mouse, sheep

species reactivity

mouse, human, frog, pig, feline, chicken, hamster, canine, bovine, fish, snake, rabbit, sheep, goat, rat, lizard

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General description

Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,β and γ subunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane.

Specificity

Reacts specifically with the α2 subunit of the DHP receptor. Strong reactivity is observed with the α2 subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines.

Immunogen

Rabbit dihydropyridine (DHP) receptors, (α2 subunit)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in:
  • immunofluorescent histochemistry
  • surface plasmon resonance binding
  • immunohistochemistry (IHC)
  • immunoblotting

Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed.

Biochem/physiol Actions

Dihydropyridine Receptor (DHFR) α1 subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide.

Other Notes

This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Upregulation of calcium channel alpha-2-delta-1 subunit in dorsal horn contributes to spinal cord injury-induced tactile allodynia
Kusuyama K, et al.
The Spine Journal : Official Journal of the North American Spine Society, 18(6), 1062-1069 (2018)
Cardiac L-type calcium channel (Cav1. 2) associates with gamma subunits
Yang L, et al.
Faseb Journal, 25(3), 928-936 (2011)
Sade Spencer et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(25), 8605-8611 (2014-06-21)
Relapse to cocaine seeking is associated with potentiated excitatory synapses in nucleus accumbens. α2δ-1 is an auxiliary subunit of voltage-gated calcium channels that affects calcium-channel trafficking and kinetics, initiates extracellular signaling cascades, and promotes excitatory synaptogenesis. Previous data demonstrate that
Damiana Scuteri et al.
Life (Basel, Switzerland), 11(1) (2021-01-16)
DYT1 dystonia is the most common form of early-onset inherited dystonia, which is caused by mutation of torsin A (TA) belonging to the "ATPases associated with a variety of cellular activities" (AAA + ATPase). Dystonia is often accompanied by pain
P Lirk et al.
British journal of anaesthesia, 113 Suppl 1, i32-i38 (2014-06-21)
Lidocaine demethylates deoxyribonucleic acid (DNA) in breast cancer cells. This modification of epigenetic information may be of therapeutic relevance in the perioperative period, because a decrease in methylation can reactivate tumour suppressor genes and inhibit tumour growth. The objectives of

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