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D4168

Sigma-Aldrich

SIGMAFAST 3,3′-Diaminobenzidine tablets

tablet, To prepare 1 mL

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CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Quality Level

form

tablet

solubility

deionized water: 1 mL, clear, pink

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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1 of 4

This Item
D4293D8001D12384
3,3′-Diaminobenzidine 97% (HPLC)

D8001

3,3′-Diaminobenzidine

3,3′-Diaminobenzidine 97%

D12384

3,3′-Diaminobenzidine

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

tablet

form

tablet

form

powder

form

powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

room temp

storage temp.

-

Application

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Reconstitution

Each tablet set dissolved in 1 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

signalword

Danger

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Ox. Liq. 3 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

5.1B - Oxidizing hazardous materials

wgk_germany

WGK 3

flash_point_f

53.6 °F - closed cup

flash_point_c

12 °C - closed cup


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Articles

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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