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D5941

Sigma-Aldrich

Drabkin′s reagent

for the determination of hemoglobin

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NACRES:
NA.25

Quality Level

form

liquid

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This Item
244023221945207810
Sigma-Aldrich

D5941

Drabkin′s reagent

-
Potassium hexacyanoferrate(III) ACS reagent, ≥99.0%

244023

Potassium hexacyanoferrate(III)

-
Potassium iodide ACS reagent, ≥99.0%

221945

Potassium iodide

Essential Grade
Potassium cyanide ACS reagent, ≥96.0%

207810

Potassium cyanide

-
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Drabkin′s reagent is used in measuring haemoglobin from blood samples. It comprises potassium ferricyanide, potassium cyanide and potassium dihydrogen phosphate as components. Potassium ferricyanide oxidizes haemoglobin to methemoglobin and then to cyanmethemoglobin. The cyanmethemoglobin levels is estimated at 530nm. It is a reliable method and can measure all haemoglobin forms except sulphaemoglobin.

Application

Drabkin′s reagent has been used for the quantitative colorimetric determination of blood haemoglobin in cerebral tissue to assess cerebral haemorrhage, in red blood cells to detect hemolysis and in contralateral and ipsilateral brain tissue homogenate.

Components

Contains sodium bicarbonate, potassium ferricyanide, and potassium cyanide.

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Danger

Hazard Classifications

Acute Tox. 2 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - STOT RE 2

target_organs

Thyroid

supp_hazards

Storage Class

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges


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Comparative measuring between fresh and stored Drabkin's reagent preparations on hemoglobin estimation
Madani N, et al.
Sudan Medical Monitor, 10(1), 7-7 (2015)
Allison Cornell et al.
The Journal of experimental biology, 220(Pt 21), 4060-4067 (2017-09-17)
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Comparative study of hemoglobin estimated by Drabkin's and Sahli's methods.
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Journal of Postgraduate Medicine, 38(1), 8-8 (1992)

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