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Key Documents

D7674

Sigma-Aldrich

6-(γ,γ-Dimethylallylamino)purine

BioReagent, suitable for plant cell culture, ≥98.5%

Synonym(s):

N6 dimethylallyladenine, 2iP, N6-(2-Isopentenyl)adenine

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About This Item

Empirical Formula (Hill Notation):
C10H13N5
CAS Number:
Molecular Weight:
203.24
MDL number:
UNSPSC Code:
10171502
PubChem Substance ID:
NACRES:
NA.72

product line

BioReagent

Quality Level

assay

≥98.5%

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

storage temp.

−20°C

SMILES string

C\C(C)=C\CNc1ncnc2[nH]cnc12

InChI

1S/C10H13N5/c1-7(2)3-4-11-9-8-10(13-5-12-8)15-6-14-9/h3,5-6H,4H2,1-2H3,(H2,11,12,13,14,15)

InChI key

HYVABZIGRDEKCD-UHFFFAOYSA-N

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Application

6-(γ,γ-Dimethylallylamino)purine (2iP) is a bacteria-derived riboside cytokinin used to grow plant tissues such as tobacco and soybean callus. 2iP is a precursor of the cytokinin Zeatin. 2iP has been used in Schenk and Hildebrandt medium to support in vitro propagation of microshoot cultures from shoot tips of Genista plants.
6-(γ,γ-Dimethylallylamino)purine has been used as a component of Murashige and Skoog medium for promoting tissue lignification in Acca sellowiana and as a component of SIM medium for used in regeneration assay of cotyledons.

Biochem/physiol Actions

6-(γ,γ-Dimethylallylamino)purine (2iP) is a bacteria-derived riboside cytokinin used to grow plant tissues such as tobacco and soybean callus. 2iP is a precursor of the cytokinin Zeatin. 2iP is used in Schenk and Hildebrandt medium to support in vitro propagation of microshoot cultures from shoot tips of Genista plants.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

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6-gamma, gamma-Dimethylallylamino) purine as a precursor of zeatin
Miura GA and Miller CO
Plant Physiology, 44(3), 372-376 (1969)
J P Helgeson et al.
Plant physiology, 44(2), 193-198 (1969-02-01)
The growth rates of tobacco callus tissues on media containing 10(-6) to 10 mum 6-(gamma,gamma-dimethylallylamino)purine (2iP) were measured. At concentrations of 10(-4) mum and above growth rates were exponential and dependent on cytokinin concentration. At 2iP concentrations of 10(-4) to
The shoot regeneration capacity of excised Arabidopsis cotyledons is established during the initial hours after injury and is modulated by a complex genetic network of light signalling
Nameth B, et al.
Plant, Cell and Environment, 36(1), 68-86 (2013)
In vitro propagation of Guayabo del pais(Acca sellowiana (Berg.) Burret)
Ross S and Grasso R
Fruit, Vegetable and Cereal Science and Biotechnology, 4, 83-87 (2010)
E Strzelczyk et al.
Acta microbiologica Polonica, 34(2), 177-185 (1985-01-01)
Studies on the effect of post culture liquids of actinomycetes on cytokinin-like substances production by mycorrhizal fungi have revealed that actinomycete metabolites inhibited or stimulated the synthesis of these compounds. The results of chromatographic analyses suggest, that substances stimulating the

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