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D8168

Sigma-Aldrich

Monoclonal Anti-Dystrophin antibody produced in mouse

clone MANDYS8, ascites fluid

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Synonym(s):
Dystrophin Antibody, Dystrophin Antibody - Monoclonal Anti-Dystrophin antibody produced in mouse
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

MANDYS8, monoclonal

species reactivity

chicken, rat, human, pig, rabbit, mouse

technique(s)

indirect ELISA: suitable
indirect immunofluorescence: 1:400 using frozen human or animal muscle tissue sections.
microarray: suitable
western blot: suitable

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)

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This Item
D8043O1136G2654
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)

Gene Information

human ... DMD(1756)
mouse ... Dmd(13405)
rat ... Dmd(24907)

Gene Information

human ... ODC1(4953)
mouse ... Odc1(18263)

Gene Information

human ... GCG(2641)
mouse ... Gcg(14526)
rat ... Gcg(24952)

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

chicken, rat, human, pig, rabbit, mouse

species reactivity

rat, human, mouse, fish

species reactivity

human, mouse

species reactivity

feline, canine, mouse, guinea pig, rat, rabbit, human, pig

clone

MANDYS8, monoclonal

clone

MANDRA1, monoclonal

clone

ODC-29, monoclonal

clone

K79bB10, monoclonal

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General description

Monoclonal Anti-Dystrophin (mouse IgG2b isotype) is derived from the MANDYS8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Dystrophin is a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like, triple-helical domain separating an N-terminal actin binding domain from two C-terminal domains, one of which is rich in cysteine.

Immunogen

recombinant human dystrophin fragment.

Application

Monoclonal Anti-Dystrophin antibody produced in mouse has been used in
  • immunohistochemistry
  • immunofluorescence
  • double immunofluorescence terminal dUTP nick-end labeling (TUNEL)
  • immunoblotting

Monoclonal anti-dystrophin antibody can be used for localization of dystrophin using immunochemical assays like ELISA and immunohistochemistry. The antibody can also be used in immunoblotting for brain dystrophin. Further, it can be used in western blotting and double immunofluorescent labelling (diluted 1: 500) of dystrophin.

Biochem/physiol Actions

Dystrophin deficiency is associated with severe Duchenne muscular dystrophy (DMD). Becker muscular dystrophy (BMD) show less pronounced abnormalities of dystrophin protein expression. Since abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases.

Target description

The rod domain of the human dystrophin molecule is present in normal muscle tissue and in nearly all Becker muscular dystrophies. It is absent in the cases of Duchenne muscular dystrophies and in the dystrophic mouse (mdx).

Other Notes

This product can be found as purified product that was produced using cell culture hybridoma product.
SAB4200764 Anti-Dystrophin antibody, Mouse monoclonal
clone MANDYS8, purified from hybridoma cell culture

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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The dystrophin complex: structure, function, and implications for therapy
Gao QQ and McNally EM
Comprehensive Physiology, 5(3), 1223-1239 (2011)
Effects of long-term resveratrol-induced SIRT1 activation on insulin and apoptotic signalling in aged skeletal muscle
Sin TK, et al.
Acta Diabetologica, 52(6), 1063-1075 (2015)
Myospryn is a calcineurin-interacting protein that negatively modulates slow-fiber-type transformation and skeletal muscle regeneration
Kielbasa OM, et al.
Faseb Journal, 25(7), 2276-2286 (2011)
Rachel Blitzblau et al.
Brain research, 1218, 21-34 (2008-06-06)
Muscular dystrophy patients often show cognitive impairment, in addition to muscle degeneration caused by dystrophin gene defects. The cognitive impairments lead to speculation that the dystrophin protein family may play a key role at neuronal synapses. Dystrophin regulates the stability
Viktoriia Kyrychenko et al.
JCI insight, 2(18) (2017-09-22)
Dystrophin maintains the integrity of striated muscles by linking the actin cytoskeleton with the cell membrane. Duchenne muscular dystrophy (DMD) is caused by mutations in the dystrophin gene (DMD) that result in progressive, debilitating muscle weakness, cardiomyopathy, and a shortened

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