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E3019

Sigma-Aldrich

Esterase from porcine liver

lyophilized powder, ≥15 units/mg solid

Synonym(s):

Carboxyl esterase, Carboxylic-ester hydrolase, PLE

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About This Item

CAS Number:
9016-18-6
Enzyme Commission number:
3.1.1.1 (BRENDA, IUBMB)
EC Number:
232-773-7
MDL number:
MFCD00131030
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

300

specific activity

≥15 units/mg solid

mol wt

168 kDa

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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This Item
46058E2884E0887
Esterase from porcine liver lyophilized powder, ≥15 units/mg solid

E3019

Esterase from porcine liver

Sigma-Aldrich

46058

Esterase from porcine liver

Esterase from porcine liver ammonium sulfate suspension, ≥150 units/mg protein (biuret)

E2884

Esterase from porcine liver

Esterase from rabbit liver lyophilized powder, ≥30 units/mg protein

E0887

Esterase from rabbit liver

specific activity

≥15 units/mg solid

specific activity

≥50 U/mg

specific activity

≥150 units/mg protein (biuret)

specific activity

≥30 units/mg protein

form

lyophilized powder

form

lyophilized solid, powder

form

ammonium sulfate suspension

form

lyophilized powder

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

-

mol wt

168 kDa

mol wt

Mr ~162000

mol wt

168 kDa

mol wt

60 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

300

Quality Level

100

Quality Level

300

Quality Level

200

Application

Esterase from porcine liver has been used in a study to assess the effect of 5-aminolaevulinic acid peptide prodrugs on photosensitization for photodynamic therapy. Esterase from porcine liver has also been used in a study to investigate how site-specific atherogenic gene expression correlates with subsequent variable lesion development in coronary and peripheral vasculature.
Porcine liver esterase is used to catalyze the hydrolysis of pentaacetyl catechin and epicatechin for use in pharmaceutical and industrial applications.

Pig liver esterase is commonly used for kinetic resolutions and assymetric synthesis in organic chemistry.
The enzyme from Sigma has been immobilised in hollow fibre ultrafiltration membrane and used for the asymmetric hydrolysis of a meso-diester.[1] Esterase from Sigma has been used to investigate its effect on the release of methacrylic acid (MAA) and 2-hydroxyethyl methacrylate (HEMA) from adhesives formulated under conditions simulating wet bonding.[2] It has been used to examine the ability of carboxylesterase activity to remove permethrin- and bifenthrin-associated toxicity to Ceriodaphnia dubia and Hyalella azteca in a variety of matrices.[3]

Biochem/physiol Actions

Esterase acts on water-soluble carboxyl esters containing short chain fatty acids. Its functionality is attributed to the catalytic triad of Ser, His and Asp/Glu.
Pig liver esterase catalyzes enantioselective conversion of an ester to a carboxylic acid. The molecular weight is found to be 168 kDa. It is a serine enzyme with two active sites on each molecule, which dissociates into active half-molecules in the presence of dilute acid or concentrated salts.[4]

Unit Definition

One unit will hydrolyze 1.0 μmole of ethyl butyrate to butyric acid and ethanol per min at pH 8.0 at 25 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000413079
0000414813
0000414813
0000413079
0000408349

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Elisabet L Kostoryz et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 88(2), 394-401 (2008-04-09)
Dentin adhesives may undergo phase separation when bonding to wet demineralized dentin. We hypothesized that adhesives exhibiting phase separation will experience enhanced biodegradation of methacrylate ester groups. The objective of this project was to study the effect of enzyme-exposure on
Craig E Wheelock et al.
Environmental toxicology and chemistry, 25(4), 973-984 (2006-04-25)
Increases in the use and application of pyrethroid insecticides have resulted in concern regarding potential effects on aquatic ecosystems. Methods for the detection of pyrethroids in receiving waters are required to monitor environmental levels of these insecticides. One method employed
Kazuhito Watanabe et al.
Biological & pharmaceutical bulletin, 28(9), 1743-1747 (2005-09-06)
The properties of ES46.5K, an esterase from mouse hepatic microsomes, were compared with those of carboxylesterases from rabbit and porcine liver. The inhibitory profile with a serine hydrolase inhibitor (bis-p-nitrophenylphosphate) and detergents (sodium dodecylsulfate, Emulgen 911) was different between ES46.5K
Anatoli Tchigvintsev et al.
Applied microbiology and biotechnology, 99(5), 2165-2178 (2014-09-10)
Most of the Earth's biosphere is cold and is populated by cold-adapted microorganisms. To explore the natural enzyme diversity of these environments and identify new carboxylesterases, we have screened three marine metagenome gene libraries for esterase activity. The screens identified
Asymmetric hydrolysis of a meso-diester using pig liver esterase immobilised in hollow fibre ultrafiltration membrane.
Sousa HA, et al.
Tetrahedron Asymmetry, 11(4), 929-934 (2000)
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Protocols

Enzymatic Assay of Esterase

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.

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